Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29144 | 3' | -54.5 | NC_006146.1 | + | 32564 | 0.66 | 0.94142 |
Target: 5'- uUGCAGGUgggCUGCauaUUCCcGcCAGCUu -3' miRNA: 3'- gACGUCCGa--GACGaa-AAGGuC-GUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 13551 | 0.66 | 0.938522 |
Target: 5'- gCUGCAGGCgccgggcgccgggCUGCgcaagcaggCCGGgGGCUc -3' miRNA: 3'- -GACGUCCGa------------GACGaaaa-----GGUCgUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 79048 | 0.66 | 0.93654 |
Target: 5'- gUGCAGGCacCUGUcuuUUUUCCAcCGGCc -3' miRNA: 3'- gACGUCCGa-GACG---AAAAGGUcGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 158612 | 0.66 | 0.93654 |
Target: 5'- -aGCAGGUUCUGgUUcagcugCCAGUAGgUg -3' miRNA: 3'- gaCGUCCGAGACgAAaa----GGUCGUCgA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 105650 | 0.66 | 0.93654 |
Target: 5'- -aGCAGGCUgagCUGUUUUgCCauuuuauucaGGCGGCUc -3' miRNA: 3'- gaCGUCCGA---GACGAAAaGG----------UCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 156782 | 0.66 | 0.931408 |
Target: 5'- gCUGUAGGC-C-GCcg--CCAGCAGCc -3' miRNA: 3'- -GACGUCCGaGaCGaaaaGGUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 93940 | 0.66 | 0.928208 |
Target: 5'- -gGCgGGGCUCcGCUcUccgucccaggcgaccUCCAGCGGCa -3' miRNA: 3'- gaCG-UCCGAGaCGAaA---------------AGGUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 46575 | 0.66 | 0.926024 |
Target: 5'- -gGguGGCg-UGCg---CCAGCAGCg -3' miRNA: 3'- gaCguCCGagACGaaaaGGUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 150178 | 0.66 | 0.920386 |
Target: 5'- -cGCGGGUccUCgcagGCcg--CCAGCAGCUu -3' miRNA: 3'- gaCGUCCG--AGa---CGaaaaGGUCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 128214 | 0.66 | 0.920386 |
Target: 5'- -gGgGGGCUCUGCcag-CCucaGGCAGCc -3' miRNA: 3'- gaCgUCCGAGACGaaaaGG---UCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 21436 | 0.66 | 0.914497 |
Target: 5'- -gGgGGaGCUCUGCUUcUCCAcGgAGCUg -3' miRNA: 3'- gaCgUC-CGAGACGAAaAGGU-CgUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 92858 | 0.68 | 0.873982 |
Target: 5'- uUGguGGCacggUCUGacUUUCCAGUGGCUg -3' miRNA: 3'- gACguCCG----AGACgaAAAGGUCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 117827 | 0.68 | 0.872484 |
Target: 5'- uCUGCAGGCUCUccacggccaccgcGUUgcUCCccaacuugaugacAGCGGCUg -3' miRNA: 3'- -GACGUCCGAGA-------------CGAaaAGG-------------UCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 10508 | 0.68 | 0.866402 |
Target: 5'- -cGCAGGCUCUGgUUUgcgagacUgGGCGGCc -3' miRNA: 3'- gaCGUCCGAGACgAAAa------GgUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 62633 | 0.68 | 0.866402 |
Target: 5'- --aCAGGCUggGC-UUUCCGGCGGCc -3' miRNA: 3'- gacGUCCGAgaCGaAAAGGUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 102039 | 0.68 | 0.850578 |
Target: 5'- -gGcCAGGCUgucgccagcCUGCUUgUCCAGCAGg- -3' miRNA: 3'- gaC-GUCCGA---------GACGAAaAGGUCGUCga -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 4810 | 0.69 | 0.816481 |
Target: 5'- aCUGuCAGGCUCcuccaGCaca-CCAGCAGCa -3' miRNA: 3'- -GAC-GUCCGAGa----CGaaaaGGUCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 20317 | 0.69 | 0.789021 |
Target: 5'- cCUGCAGGCgggUGCUggacgauggCCAGCAGa- -3' miRNA: 3'- -GACGUCCGag-ACGAaaa------GGUCGUCga -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 104552 | 0.71 | 0.689285 |
Target: 5'- cCUGCaccagGGGCUCgaugcUGCUgucaUUCCAGUGGCUa -3' miRNA: 3'- -GACG-----UCCGAG-----ACGAa---AAGGUCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 115593 | 0.72 | 0.668417 |
Target: 5'- cCUGuCAGGCUucgccCUGCacuUUUUCCGGCuGCUg -3' miRNA: 3'- -GAC-GUCCGA-----GACG---AAAAGGUCGuCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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