miRNA: 3'- gACGUCC-GAGACGaaaAGG--UCGUCGa -5'
miRNA: 3'- -GAC--GUCCGAGACgAAAAGGUCG------------UCGa -5'
miRNA: 3'- -GACGUCCGAGACGAaAAGGuCGuCGa -5'
miRNA: 3'- -GACGUCCGAGa--CGAaaaGGUCGUCGA- -5'
miRNA: 3'- -GACGUCCGAGACGAAAAGGUCGUCGA- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29144 | 3' | -54.5 | NC_006146.1 | + | 131197 | 0.73 | 0.605356 |
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Target: 5'- gUGCAGGcCUCUGCc--UCCggAGCGGCg -3' miRNA: 3'- gACGUCC-GAGACGaaaAGG--UCGUCGa -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 50339 | 0.74 | 0.553294 |
|
Target: 5'- uCUGacCAGGCUCUGgUUUUCCAGCccguggagauccAGCg -3' miRNA: 3'- -GAC--GUCCGAGACgAAAAGGUCG------------UCGa -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 1699 | 0.76 | 0.426259 |
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Target: 5'- uCUGCAGGCUCUGCUgUUUCuGCuGUc -3' miRNA: 3'- -GACGUCCGAGACGAaAAGGuCGuCGa -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 132787 | 0.81 | 0.21522 |
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Target: 5'- gCUGCAGGCUCgcaGCUgc-UCAGCAGCUg -3' miRNA: 3'- -GACGUCCGAGa--CGAaaaGGUCGUCGA- -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 159497 | 1.07 | 0.004162 |
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Target: 5'- cCUGCAGGCUCUGCUUUUCCAGCAGCUc -3' miRNA: 3'- -GACGUCCGAGACGAAAAGGUCGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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