Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29144 | 3' | -54.5 | NC_006146.1 | + | 150178 | 0.66 | 0.920386 |
Target: 5'- -cGCGGGUccUCgcagGCcg--CCAGCAGCUu -3' miRNA: 3'- gaCGUCCG--AGa---CGaaaaGGUCGUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 13551 | 0.66 | 0.938522 |
Target: 5'- gCUGCAGGCgccgggcgccgggCUGCgcaagcaggCCGGgGGCUc -3' miRNA: 3'- -GACGUCCGa------------GACGaaaa-----GGUCgUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 21436 | 0.66 | 0.914497 |
Target: 5'- -gGgGGaGCUCUGCUUcUCCAcGgAGCUg -3' miRNA: 3'- gaCgUC-CGAGACGAAaAGGU-CgUCGA- -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 128214 | 0.66 | 0.920386 |
Target: 5'- -gGgGGGCUCUGCcag-CCucaGGCAGCc -3' miRNA: 3'- gaCgUCCGAGACGaaaaGG---UCGUCGa -5' |
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29144 | 3' | -54.5 | NC_006146.1 | + | 32564 | 0.66 | 0.94142 |
Target: 5'- uUGCAGGUgggCUGCauaUUCCcGcCAGCUu -3' miRNA: 3'- gACGUCCGa--GACGaa-AAGGuC-GUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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