Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29147 | 3' | -53.6 | NC_006146.1 | + | 45419 | 0.7 | 0.868944 |
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Target: 5'- cCAGCGCGagGCCC-CGGCCGg--GUUg -3' miRNA: 3'- aGUUGCGCa-UGGGcGCUGGCauaCAG- -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 11521 | 0.72 | 0.803336 |
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Target: 5'- aCAGCGCG-GCCUGCGAgCUGgcgGUCa -3' miRNA: 3'- aGUUGCGCaUGGGCGCU-GGCauaCAG- -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 167648 | 0.72 | 0.785222 |
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Target: 5'- cCGGCGCGUGCCgGgGGCCcGggggcGUGUCc -3' miRNA: 3'- aGUUGCGCAUGGgCgCUGG-Ca----UACAG- -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 170356 | 0.72 | 0.766557 |
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Target: 5'- --cACGUGUggagGCCCGCGgagagGCCGUGUGUg -3' miRNA: 3'- aguUGCGCA----UGGGCGC-----UGGCAUACAg -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 168493 | 0.72 | 0.766557 |
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Target: 5'- --cACGUGUggagGCCCGCGgagagGCCGUGUGUg -3' miRNA: 3'- aguUGCGCA----UGGGCGC-----UGGCAUACAg -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 167561 | 0.72 | 0.766557 |
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Target: 5'- --cACGUGUggagGCCCGCGgagagGCCGUGUGUg -3' miRNA: 3'- aguUGCGCA----UGGGCGC-----UGGCAUACAg -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 169425 | 0.72 | 0.766557 |
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Target: 5'- --cACGUGUggagGCCCGCGgagagGCCGUGUGUg -3' miRNA: 3'- aguUGCGCA----UGGGCGC-----UGGCAUACAg -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 166520 | 0.75 | 0.606124 |
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Target: 5'- uUCAugGCGUcggccuggggaGCCCGCGggGCCGUGgccacGUCg -3' miRNA: 3'- -AGUugCGCA-----------UGGGCGC--UGGCAUa----CAG- -5' |
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| 29147 | 3' | -53.6 | NC_006146.1 | + | 161400 | 1.09 | 0.005684 |
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Target: 5'- gUCAACGCGUACCCGCGACCGUAUGUCc -3' miRNA: 3'- -AGUUGCGCAUGGGCGCUGGCAUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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