Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29169 | 5' | -58.2 | NC_006150.1 | + | 182555 | 0.67 | 0.845746 |
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Target: 5'- gGGGgGCaccCGGGGGGuUCGCgGAGCGGg -3' miRNA: 3'- -CCUgCGc--GCCCUCU-AGCGgUUCGCUg -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 113904 | 0.68 | 0.837997 |
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Target: 5'- gGGAC-CGCGGGGccUCGCCA-GCcGCa -3' miRNA: 3'- -CCUGcGCGCCCUcuAGCGGUuCGcUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 182606 | 0.68 | 0.837997 |
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Target: 5'- -aGCGCaGCGGGuGGUCGCaCAGGagggaGGCc -3' miRNA: 3'- ccUGCG-CGCCCuCUAGCG-GUUCg----CUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 49824 | 0.68 | 0.821994 |
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Target: 5'- -uGCGCGaCGGGAGAuUCGC---GCGGCu -3' miRNA: 3'- ccUGCGC-GCCCUCU-AGCGguuCGCUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 1030 | 0.68 | 0.796827 |
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Target: 5'- cGGCGCGCaggcaGGcGAGGguggccugaCGCUAGGCGGCg -3' miRNA: 3'- cCUGCGCG-----CC-CUCUa--------GCGGUUCGCUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 148971 | 0.68 | 0.796827 |
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Target: 5'- -cACGUGCGGGAGAaggCGCCcugGAGCc-- -3' miRNA: 3'- ccUGCGCGCCCUCUa--GCGG---UUCGcug -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 1737 | 0.69 | 0.752273 |
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Target: 5'- gGGugGCGgcggaGGGGGGUUGgCG-GCGGCa -3' miRNA: 3'- -CCugCGCg----CCCUCUAGCgGUuCGCUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 59744 | 1.1 | 0.002566 |
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Target: 5'- uGGACGCGCGGGAGAUCGCCAAGCGACc -3' miRNA: 3'- -CCUGCGCGCCCUCUAGCGGUUCGCUG- -5' |
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| 29169 | 5' | -58.2 | NC_006150.1 | + | 119800 | 0.66 | 0.906315 |
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Target: 5'- gGGGCcgcaGCGCGGuaGAGcgCGCCAAagacgccGCGAa -3' miRNA: 3'- -CCUG----CGCGCC--CUCuaGCGGUU-------CGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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