Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29176 | 3' | -66.5 | NC_006150.1 | + | 102349 | 0.66 | 0.57459 |
Target: 5'- aGGCG-GCGCUUCggGCCCaCCGCCAGa- -3' miRNA: 3'- -UCGCgUGCGAGG--CGGGgGGCGGUCcc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 1847 | 0.66 | 0.556285 |
Target: 5'- cAGCGCGCGCUCgcgacgGCCCCaucaaaagcggCgGCUGGGa -3' miRNA: 3'- -UCGCGUGCGAGg-----CGGGG-----------GgCGGUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 206388 | 0.66 | 0.538153 |
Target: 5'- uGCGCGCGCUCC-UCCCCCaUCAa-- -3' miRNA: 3'- uCGCGUGCGAGGcGGGGGGcGGUccc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 125649 | 0.66 | 0.529162 |
Target: 5'- aAGCGCgaaacgugGCGCgCCGCCaucuCCCuaCGGGGu -3' miRNA: 3'- -UCGCG--------UGCGaGGCGGg---GGGcgGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 182090 | 0.67 | 0.511352 |
Target: 5'- aGGCGaaCAUGUUCgGCCCgaaCCCGCUGGGc -3' miRNA: 3'- -UCGC--GUGCGAGgCGGG---GGGCGGUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 88183 | 0.67 | 0.502541 |
Target: 5'- gGGcCGCGCGCccCCGCCCuucugggcggCCCuCUGGGGg -3' miRNA: 3'- -UC-GCGUGCGa-GGCGGG----------GGGcGGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 204911 | 0.67 | 0.502541 |
Target: 5'- -aCGgaGCGUUuuGaCCCUCGCCAGGGg -3' miRNA: 3'- ucGCg-UGCGAggCgGGGGGCGGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 106395 | 0.67 | 0.467996 |
Target: 5'- cGUGCccCGuCUCCGCCCCaCGCUgcaaAGGGc -3' miRNA: 3'- uCGCGu-GC-GAGGCGGGGgGCGG----UCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 150241 | 0.67 | 0.459551 |
Target: 5'- gAGCGCcagcggggugGCGUUCgGCaCCCUGCCcGGGc -3' miRNA: 3'- -UCGCG----------UGCGAGgCGgGGGGCGGuCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 88457 | 0.67 | 0.458711 |
Target: 5'- cGCGCAUgGCUaCGCCCCcagagggCCGCCcagaAGGGc -3' miRNA: 3'- uCGCGUG-CGAgGCGGGG-------GGCGG----UCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 115173 | 0.68 | 0.418594 |
Target: 5'- gGGCGaUGCGCUCUGCcaacuaCCCCCGCUcuuuucGGGc -3' miRNA: 3'- -UCGC-GUGCGAGGCG------GGGGGCGGu-----CCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 758 | 0.68 | 0.410672 |
Target: 5'- uGGCGCuCGCUgCGCCgCCUagCGUCAGGc -3' miRNA: 3'- -UCGCGuGCGAgGCGG-GGG--GCGGUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 130583 | 0.69 | 0.387473 |
Target: 5'- aAGCGCACcaCUCCGCcuuCCCCCGCUg--- -3' miRNA: 3'- -UCGCGUGc-GAGGCG---GGGGGCGGuccc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 88273 | 0.69 | 0.379934 |
Target: 5'- uAGCGCcaGCGgaacgCCGCCCCaucccccaggCgGCCAGGGg -3' miRNA: 3'- -UCGCG--UGCga---GGCGGGG----------GgCGGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 33156 | 0.69 | 0.365154 |
Target: 5'- cGCGCcgaccGCGCUCCG-CCUCCGCUucGGa -3' miRNA: 3'- uCGCG-----UGCGAGGCgGGGGGCGGucCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 149585 | 0.7 | 0.350775 |
Target: 5'- aGGCgGUACGCUUCuCCUCCgGCUGGGGc -3' miRNA: 3'- -UCG-CGUGCGAGGcGGGGGgCGGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 85683 | 0.7 | 0.343738 |
Target: 5'- cGGUGgGCGCUCgGCCCUCC-UCAGGc -3' miRNA: 3'- -UCGCgUGCGAGgCGGGGGGcGGUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 129282 | 0.7 | 0.323242 |
Target: 5'- -aCGUACGCUCCGCCucugCCCCGacugCAGGc -3' miRNA: 3'- ucGCGUGCGAGGCGG----GGGGCg---GUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 82671 | 0.7 | 0.316615 |
Target: 5'- --aGUugGCUaCCGCCuCCCCGacagCAGGGg -3' miRNA: 3'- ucgCGugCGA-GGCGG-GGGGCg---GUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 62095 | 0.71 | 0.291137 |
Target: 5'- uGCGCACGCaucgguccguaUCCGCCauaCCCGCUucGGc -3' miRNA: 3'- uCGCGUGCG-----------AGGCGGg--GGGCGGucCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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