miRNA: 3'- uCGCGugCGa--GgCGGGgGGCGGuCCC- -5'
miRNA: 3'- -UCGCGUGCGAG---GCGGGGGGCGGU--------CCC- -5'
miRNA: 3'- -UCGCGUGCGa-----GGC------GGGGGGC-GGUCCC- -5'
miRNA: 3'- -UCGCGUGCGAGGCGGGGGGCGGUCCC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29176 | 3' | -66.5 | NC_006150.1 | + | 139381 | 0.72 | 0.261582 |
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Target: 5'- uGCGCugGCggaCgGUCCgCCGCCuGGGg -3' miRNA: 3'- uCGCGugCGa--GgCGGGgGGCGGuCCC- -5' |
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| 29176 | 3' | -66.5 | NC_006150.1 | + | 718 | 0.74 | 0.191745 |
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Target: 5'- cGGCGCGUGUUUugaCGCCCCCCGCCGcgacccccGGGc -3' miRNA: 3'- -UCGCGUGCGAG---GCGGGGGGCGGU--------CCC- -5' |
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| 29176 | 3' | -66.5 | NC_006150.1 | + | 88343 | 0.78 | 0.092667 |
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Target: 5'- cGGCGCAUGCaggacgCCGucccauCCCCCCGaCCAGGGg -3' miRNA: 3'- -UCGCGUGCGa-----GGC------GGGGGGC-GGUCCC- -5' |
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| 29176 | 3' | -66.5 | NC_006150.1 | + | 88507 | 1.08 | 0.000669 |
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Target: 5'- gAGCGCACGCUCCGCCCCCCGCCAGGGc -3' miRNA: 3'- -UCGCGUGCGAGGCGGGGGGCGGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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