Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29177 | 3' | -57.8 | NC_006150.1 | + | 128451 | 0.68 | 0.795528 |
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Target: 5'- aGAUggCGGCUuuGCCACCAUGGaaggcgacGCGAAAc -3' miRNA: 3'- -CUGuaGCCGG--CGGUGGUGCC--------UGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 31858 | 0.69 | 0.731242 |
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Target: 5'- aGGCAgaacacgCGGCCGCCAuCCACcaGGACa--- -3' miRNA: 3'- -CUGUa------GCCGGCGGU-GGUG--CCUGcuuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 94798 | 0.7 | 0.718781 |
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Target: 5'- aGACAaaaaccauuguuccUgGGCCGCCAucUCGCGGAUGAu- -3' miRNA: 3'- -CUGU--------------AgCCGGCGGU--GGUGCCUGCUuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 118355 | 0.7 | 0.712021 |
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Target: 5'- aGCAg-GGCUuaCACCACGGugGAAAa -3' miRNA: 3'- cUGUagCCGGcgGUGGUGCCugCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 107685 | 0.7 | 0.682729 |
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Target: 5'- uGugGUCGGCCGCUgggggaGCgACGGcCGggGa -3' miRNA: 3'- -CugUAGCCGGCGG------UGgUGCCuGCuuU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 106266 | 0.71 | 0.64316 |
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Target: 5'- cGGCAaCaGCCGCgGCCGCGGGgGGAGa -3' miRNA: 3'- -CUGUaGcCGGCGgUGGUGCCUgCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 139386 | 0.71 | 0.623295 |
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Target: 5'- uGGCGgaCGGuCCGCCGCCugGGguACGGGAg -3' miRNA: 3'- -CUGUa-GCC-GGCGGUGGugCC--UGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 89024 | 1.06 | 0.004518 |
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Target: 5'- cGACAUCGGCCGCCACCACGGACGAAAu -3' miRNA: 3'- -CUGUAGCCGGCGGUGGUGCCUGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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