miRNA: 3'- -GCCUgCGCCU----UGCCGG-GCUGAaUGGC- -5'
miRNA: 3'- gCCUGCGccuu-GCCG-GGCUGa-AUGGC- -5'
miRNA: 3'- gCCUGCGCCU-------UgCCGGGcUGa-AUGGC- -5'
miRNA: 3'- -GCCugCgCCUUgCCGGGCuGAAuGGC- -5'
miRNA: 3'- gCCUgCgCCUUgCCGG-GCugAA--------UGGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29232 | 3' | -58 | NC_006150.1 | + | 202986 | 0.66 | 0.919246 |
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Target: 5'- gCGGAgGCGGAggcgACGGCUgUGGCU-GCUGu -3' miRNA: 3'- -GCCUgCGCCU----UGCCGG-GCUGAaUGGC- -5' |
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| 29232 | 3' | -58 | NC_006150.1 | + | 140906 | 0.66 | 0.924491 |
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Target: 5'- uGGACGUcuugcCGGCaCCGGCcgUGCCGg -3' miRNA: 3'- gCCUGCGccuu-GCCG-GGCUGa-AUGGC- -5' |
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| 29232 | 3' | -58 | NC_006150.1 | + | 180508 | 0.66 | 0.929027 |
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Target: 5'- aGGACGCGGGgguagagAgGGCCUaGCagUACUGa -3' miRNA: 3'- gCCUGCGCCU-------UgCCGGGcUGa-AUGGC- -5' |
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| 29232 | 3' | -58 | NC_006150.1 | + | 132458 | 0.66 | 0.929521 |
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Target: 5'- gCGGuuGgGGAAaGGCCCG-CUUuCCGa -3' miRNA: 3'- -GCCugCgCCUUgCCGGGCuGAAuGGC- -5' |
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| 29232 | 3' | -58 | NC_006150.1 | + | 593 | 0.66 | 0.929521 |
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Target: 5'- gGGAgGgGGAAgGGCCgCGugUUcuuggcagACCGc -3' miRNA: 3'- gCCUgCgCCUUgCCGG-GCugAA--------UGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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