Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29361 | 3' | -63.8 | NC_006150.1 | + | 219993 | 0.68 | 0.498107 |
Target: 5'- -gUCCUGCCGc-CGCCGCuGCUGCUg -3' miRNA: 3'- caGGGACGGCucGUGGCGuCGGCGGu -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 170283 | 0.7 | 0.420237 |
Target: 5'- --gCCUGCaCGccgguaucGGCGCCGCgAGCCGCUAu -3' miRNA: 3'- cagGGACG-GC--------UCGUGGCG-UCGGCGGU- -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 2232 | 0.7 | 0.388064 |
Target: 5'- uUCagCUGCaggCGAGgGCCGUGGCCGCCGa -3' miRNA: 3'- cAGg-GACG---GCUCgUGGCGUCGGCGGU- -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 150216 | 0.72 | 0.28886 |
Target: 5'- -aCCCUGcCCGGGCAggUCGC-GCCGCCc -3' miRNA: 3'- caGGGAC-GGCUCGU--GGCGuCGGCGGu -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 181103 | 0.72 | 0.28886 |
Target: 5'- -cCCCUGuCCGAGCgacgccgacGCCGCuguugcucaGGCCGCCu -3' miRNA: 3'- caGGGAC-GGCUCG---------UGGCG---------UCGGCGGu -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 195291 | 0.75 | 0.205874 |
Target: 5'- -aUCCUGCaGAGCGCCGCGGCUGUg- -3' miRNA: 3'- caGGGACGgCUCGUGGCGUCGGCGgu -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 88492 | 0.75 | 0.187529 |
Target: 5'- -cCCCcGCCaGGGCugCGCGGCCGCUc -3' miRNA: 3'- caGGGaCGG-CUCGugGCGUCGGCGGu -5' |
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29361 | 3' | -63.8 | NC_006150.1 | + | 152648 | 0.66 | 0.648796 |
Target: 5'- aGUCCCgcacaccCCGcAG-GCCGUGGCCGCCu -3' miRNA: 3'- -CAGGGac-----GGC-UCgUGGCGUCGGCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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