Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29371 | 5' | -53.8 | NC_006151.1 | + | 101420 | 0.66 | 0.939946 |
Target: 5'- cGCAGC-GCGCCGAGUccuUCaCgCUGGUc -3' miRNA: 3'- aCGUCGaUGUGGUUCA---AGcG-GACCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 53024 | 0.66 | 0.939946 |
Target: 5'- -cCAGCc-CugCAGGUUCGCCgGGUu -3' miRNA: 3'- acGUCGauGugGUUCAAGCGGaCCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 24738 | 0.66 | 0.934926 |
Target: 5'- gUGCGGCaGgACCAGGcacgUCGCCgGGc- -3' miRNA: 3'- -ACGUCGaUgUGGUUCa---AGCGGaCCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 91304 | 0.66 | 0.933891 |
Target: 5'- aGCAGCcGCGCCGGGaaggCGCCguacaccucgaaGGUGa -3' miRNA: 3'- aCGUCGaUGUGGUUCaa--GCGGa-----------CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 94399 | 0.66 | 0.929647 |
Target: 5'- gGCAGCUcguGCagACCAGGUcccCGCC-GGUGa -3' miRNA: 3'- aCGUCGA---UG--UGGUUCAa--GCGGaCCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 50227 | 0.66 | 0.928013 |
Target: 5'- gUGCGGCUGCACCGGGacaacguguuugcggCGCCa---- -3' miRNA: 3'- -ACGUCGAUGUGGUUCaa-------------GCGGaccau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 84336 | 0.66 | 0.92411 |
Target: 5'- cGCGGCcuCGCCGAGUacgUCGCCggcccgcggcGGUGa -3' miRNA: 3'- aCGUCGauGUGGUUCA---AGCGGa---------CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 3276 | 0.66 | 0.92411 |
Target: 5'- cGCGGCgaugUGCGCCAGGgcgGCCgGGUc -3' miRNA: 3'- aCGUCG----AUGUGGUUCaagCGGaCCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 118137 | 0.67 | 0.912258 |
Target: 5'- cGCAGCgccgacgACGCCGcggcGGUggCGCCgcUGGUGc -3' miRNA: 3'- aCGUCGa------UGUGGU----UCAa-GCGG--ACCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 95217 | 0.67 | 0.910391 |
Target: 5'- aGCGGCUGCGCCGcacgcccucgaccgAGg-CGCCcgucgGGUAg -3' miRNA: 3'- aCGUCGAUGUGGU--------------UCaaGCGGa----CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 106763 | 0.67 | 0.905946 |
Target: 5'- aGCAGCgcgACGCgCGuGagCGCCUGGa- -3' miRNA: 3'- aCGUCGa--UGUG-GUuCaaGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 106633 | 0.67 | 0.892563 |
Target: 5'- cGCGGU--CACCGAG--CGCCUGGa- -3' miRNA: 3'- aCGUCGauGUGGUUCaaGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 120104 | 0.67 | 0.885499 |
Target: 5'- cGguGCUGCGCCGc---CGCgUGGUGg -3' miRNA: 3'- aCguCGAUGUGGUucaaGCGgACCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 2453 | 0.68 | 0.870647 |
Target: 5'- cGCAGCgggGCGCCGagcccccagcGGUUgGCCgcgcGGUGc -3' miRNA: 3'- aCGUCGa--UGUGGU----------UCAAgCGGa---CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 48938 | 0.68 | 0.843299 |
Target: 5'- aGguGCUGC-CCGcgcgcgaggacguGUUCGCCUGGa- -3' miRNA: 3'- aCguCGAUGuGGUu------------CAAGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 81395 | 0.69 | 0.82075 |
Target: 5'- cGCGGC-GC-CCAGGUUgGCCagGGUGg -3' miRNA: 3'- aCGUCGaUGuGGUUCAAgCGGa-CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 137102 | 0.7 | 0.774004 |
Target: 5'- cGguGCUG-GCCGGGgaCGCCUGGg- -3' miRNA: 3'- aCguCGAUgUGGUUCaaGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 81940 | 0.7 | 0.774004 |
Target: 5'- gGCGGCcACGCCGAGgcgcacCGCCgcgcGGUAa -3' miRNA: 3'- aCGUCGaUGUGGUUCaa----GCGGa---CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 72745 | 0.7 | 0.75826 |
Target: 5'- cGCAGCaGCGCCGAG-UCGCUggcgcacagcggcgGGUAc -3' miRNA: 3'- aCGUCGaUGUGGUUCaAGCGGa-------------CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 127850 | 0.7 | 0.754274 |
Target: 5'- cGCGGCUGCGCCGgagGGggCGCCg---- -3' miRNA: 3'- aCGUCGAUGUGGU---UCaaGCGGaccau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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