Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29371 | 5' | -53.8 | NC_006151.1 | + | 103311 | 0.71 | 0.703089 |
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Target: 5'- cGCGGCgcgACGCCGAGcugCGCacgCUGGUGc -3' miRNA: 3'- aCGUCGa--UGUGGUUCaa-GCG---GACCAU- -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 99955 | 0.71 | 0.692617 |
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Target: 5'- aGCGGCUaccGCACCAAGcUCGCCg---- -3' miRNA: 3'- aCGUCGA---UGUGGUUCaAGCGGaccau -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 71332 | 0.72 | 0.639631 |
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Target: 5'- gGCGGCUGCGCCGAcgaccUCGCCgUGGc- -3' miRNA: 3'- aCGUCGAUGUGGUUca---AGCGG-ACCau -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 85807 | 0.77 | 0.381627 |
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Target: 5'- gUGCAGCgcCACCAgcucaaccGGUgCGCCUGGUGg -3' miRNA: 3'- -ACGUCGauGUGGU--------UCAaGCGGACCAU- -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 105235 | 0.79 | 0.280974 |
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Target: 5'- aGCGGCUGacggcCGCCGAGgUCGCCUGGg- -3' miRNA: 3'- aCGUCGAU-----GUGGUUCaAGCGGACCau -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 138942 | 1.07 | 0.004796 |
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Target: 5'- cUGCAGCUACACCAAGUUCGCCUGGUAc -3' miRNA: 3'- -ACGUCGAUGUGGUUCAAGCGGACCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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