Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29371 | 5' | -53.8 | NC_006151.1 | + | 50227 | 0.66 | 0.928013 |
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Target: 5'- gUGCGGCUGCACCGGGacaacguguuugcggCGCCa---- -3' miRNA: 3'- -ACGUCGAUGUGGUUCaa-------------GCGGaccau -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 94399 | 0.66 | 0.929647 |
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Target: 5'- gGCAGCUcguGCagACCAGGUcccCGCC-GGUGa -3' miRNA: 3'- aCGUCGA---UG--UGGUUCAa--GCGGaCCAU- -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 91304 | 0.66 | 0.933891 |
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Target: 5'- aGCAGCcGCGCCGGGaaggCGCCguacaccucgaaGGUGa -3' miRNA: 3'- aCGUCGaUGUGGUUCaa--GCGGa-----------CCAU- -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 24738 | 0.66 | 0.934926 |
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Target: 5'- gUGCGGCaGgACCAGGcacgUCGCCgGGc- -3' miRNA: 3'- -ACGUCGaUgUGGUUCa---AGCGGaCCau -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 101420 | 0.66 | 0.939946 |
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Target: 5'- cGCAGC-GCGCCGAGUccuUCaCgCUGGUc -3' miRNA: 3'- aCGUCGaUGUGGUUCA---AGcG-GACCAu -5' |
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| 29371 | 5' | -53.8 | NC_006151.1 | + | 53024 | 0.66 | 0.939946 |
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Target: 5'- -cCAGCc-CugCAGGUUCGCCgGGUu -3' miRNA: 3'- acGUCGauGugGUUCAAGCGGaCCAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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