Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29371 | 5' | -53.8 | NC_006151.1 | + | 3276 | 0.66 | 0.92411 |
Target: 5'- cGCGGCgaugUGCGCCAGGgcgGCCgGGUc -3' miRNA: 3'- aCGUCG----AUGUGGUUCaagCGGaCCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 118137 | 0.67 | 0.912258 |
Target: 5'- cGCAGCgccgacgACGCCGcggcGGUggCGCCgcUGGUGc -3' miRNA: 3'- aCGUCGa------UGUGGU----UCAa-GCGG--ACCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 95217 | 0.67 | 0.910391 |
Target: 5'- aGCGGCUGCGCCGcacgcccucgaccgAGg-CGCCcgucgGGUAg -3' miRNA: 3'- aCGUCGAUGUGGU--------------UCaaGCGGa----CCAU- -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 106763 | 0.67 | 0.905946 |
Target: 5'- aGCAGCgcgACGCgCGuGagCGCCUGGa- -3' miRNA: 3'- aCGUCGa--UGUG-GUuCaaGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 106633 | 0.67 | 0.892563 |
Target: 5'- cGCGGU--CACCGAG--CGCCUGGa- -3' miRNA: 3'- aCGUCGauGUGGUUCaaGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 138942 | 1.07 | 0.004796 |
Target: 5'- cUGCAGCUACACCAAGUUCGCCUGGUAc -3' miRNA: 3'- -ACGUCGAUGUGGUUCAAGCGGACCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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