Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29387 | 3' | -53.2 | NC_006151.1 | + | 119404 | 0.69 | 0.861828 |
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Target: 5'- ---gGCGCGCgUGCuGUUCuACCUggaGCGc -3' miRNA: 3'- caaaCGCGCGgACGuCAAG-UGGA---UGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 119545 | 0.66 | 0.948142 |
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Target: 5'- --cUGCGCGCacacaaccCUGCAc--CGCCUGCGc -3' miRNA: 3'- caaACGCGCG--------GACGUcaaGUGGAUGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 125327 | 1.1 | 0.003987 |
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Target: 5'- aGUUUGCGCGCCUGCAGUUCACCUACGa -3' miRNA: 3'- -CAAACGCGCGGACGUCAAGUGGAUGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 127803 | 0.72 | 0.717571 |
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Target: 5'- ---cGCGCGCgggggcgcgagguccUUGCGGUUCACgUACGu -3' miRNA: 3'- caaaCGCGCG---------------GACGUCAAGUGgAUGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 130140 | 0.71 | 0.763773 |
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Target: 5'- ---cGCGCGCCaGCAGguaCACCUcugccACGg -3' miRNA: 3'- caaaCGCGCGGaCGUCaa-GUGGA-----UGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 132437 | 0.68 | 0.895534 |
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Target: 5'- ---gGCGCGCCUGCGcGUgcagguagcgcagCACCgcGCGg -3' miRNA: 3'- caaaCGCGCGGACGU-CAa------------GUGGa-UGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 137878 | 0.66 | 0.963833 |
|
Target: 5'- ---gGCGCaGCCUGCGGcugcccuucUUCGCCaaggucgACGg -3' miRNA: 3'- caaaCGCG-CGGACGUC---------AAGUGGa------UGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 139955 | 0.7 | 0.828708 |
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Target: 5'- aGUUcG-GCGCCgaGCAGUUCACCccggACGg -3' miRNA: 3'- -CAAaCgCGCGGa-CGUCAAGUGGa---UGC- -5' |
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| 29387 | 3' | -53.2 | NC_006151.1 | + | 140303 | 0.69 | 0.868809 |
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Target: 5'- ---gGCGcCGCCUGCGGgacgugcgcugcaUcgUCACCUACa -3' miRNA: 3'- caaaCGC-GCGGACGUC-------------A--AGUGGAUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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