Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29405 | 5' | -54.6 | NC_006151.1 | + | 125780 | 0.77 | 0.385056 |
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Target: 5'- cGUGCGCaugguggaGGUGCCCGAGAcgaucagcacGCGGGUGAc -3' miRNA: 3'- -CAUGCG--------CUACGGGCUCUu---------CGCCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 127264 | 0.71 | 0.728909 |
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Target: 5'- -cGCGCGGUGgCCGGuGAGGUcGAUGAc -3' miRNA: 3'- caUGCGCUACgGGCU-CUUCGcCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 130270 | 0.67 | 0.911807 |
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Target: 5'- -gGCGCGcgGcCCCGAGGccGCGGGc-- -3' miRNA: 3'- caUGCGCuaC-GGGCUCUu-CGCCUacu -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 134192 | 0.71 | 0.728909 |
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Target: 5'- aUACGCGAUGCUCGucgcgcagcgcGGggGCGGu--- -3' miRNA: 3'- cAUGCGCUACGGGC-----------UCuuCGCCuacu -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 138244 | 0.67 | 0.885717 |
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Target: 5'- aGUACGCGG-GCgCGAccgcGcAGCGGGUGGu -3' miRNA: 3'- -CAUGCGCUaCGgGCU----CuUCGCCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 140391 | 0.67 | 0.899238 |
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Target: 5'- cUGCGUGcUGCCCGAGGaccuGGgGGAc-- -3' miRNA: 3'- cAUGCGCuACGGGCUCU----UCgCCUacu -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 141310 | 0.66 | 0.925604 |
|
Target: 5'- cGUACGCGGgcggGUCCGGGAuggucaccgcguacGCGGgcGGg -3' miRNA: 3'- -CAUGCGCUa---CGGGCUCUu-------------CGCCuaCU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 141713 | 0.79 | 0.292201 |
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Target: 5'- -gACGCGGUGCCCGGGGcguuaauacccagGGCGGAgGGg -3' miRNA: 3'- caUGCGCUACGGGCUCU-------------UCGCCUaCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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