Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29405 | 5' | -54.6 | NC_006151.1 | + | 56313 | 0.74 | 0.544109 |
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Target: 5'- -cGCGCGGUGCCUGAGccuGGGCcGGUGGc -3' miRNA: 3'- caUGCGCUACGGGCUC---UUCGcCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 72554 | 0.74 | 0.544109 |
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Target: 5'- -cACGCGcgGCCgCGcgggaggguGGggGCGGGUGAc -3' miRNA: 3'- caUGCGCuaCGG-GC---------UCuuCGCCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 2485 | 0.74 | 0.524031 |
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Target: 5'- -cGCGCGGUGCCCGA-AGGCGGc--- -3' miRNA: 3'- caUGCGCUACGGGCUcUUCGCCuacu -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 5251 | 0.75 | 0.475191 |
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Target: 5'- -gGCgGCGGggGCCCGGGggGCGGAg-- -3' miRNA: 3'- caUG-CGCUa-CGGGCUCuuCGCCUacu -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 125780 | 0.77 | 0.385056 |
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Target: 5'- cGUGCGCaugguggaGGUGCCCGAGAcgaucagcacGCGGGUGAc -3' miRNA: 3'- -CAUGCG--------CUACGGGCUCUu---------CGCCUACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 121512 | 0.78 | 0.360303 |
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Target: 5'- -gGCGCcccGCCCGAGAAGCGGcUGAc -3' miRNA: 3'- caUGCGcuaCGGGCUCUUCGCCuACU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 141713 | 0.79 | 0.292201 |
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Target: 5'- -gACGCGGUGCCCGGGGcguuaauacccagGGCGGAgGGg -3' miRNA: 3'- caUGCGCUACGGGCUCU-------------UCGCCUaCU- -5' |
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| 29405 | 5' | -54.6 | NC_006151.1 | + | 115494 | 1.09 | 0.003384 |
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Target: 5'- cGUACGCGAUGCCCGAGAAGCGGAUGAa -3' miRNA: 3'- -CAUGCGCUACGGGCUCUUCGCCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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