miRNA: 3'- -UG-UCCUACaCgaCuuuCACGCGCGGc -5'
miRNA: 3'- ugUCCUACACGacuuuCACGCgCGGC- -5'
miRNA: 3'- -UGUCCUa---Ca---CGaCUUUCa----CGCGCGGC- -5'
miRNA: 3'- -UGuCCUa----CAC---GACUUUCACG-CGCGGC- -5'
miRNA: 3'- -UGUCCuaca-CGaCuuUCACGCGCGGc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29446 | 3' | -54.7 | NC_006151.1 | + | 89620 | 0.66 | 0.925443 |
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Target: 5'- cGCgAGGAUGaGgaGcguGUGCGCGCCc -3' miRNA: 3'- -UG-UCCUACaCgaCuuuCACGCGCGGc -5' |
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| 29446 | 3' | -54.7 | NC_006151.1 | + | 42673 | 0.66 | 0.925443 |
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Target: 5'- -gGGGAUGUGUgc---GUGCGaGCCGg -3' miRNA: 3'- ugUCCUACACGacuuuCACGCgCGGC- -5' |
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| 29446 | 3' | -54.7 | NC_006151.1 | + | 29446 | 0.66 | 0.925443 |
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Target: 5'- gGCGGGGagagGggacGCgGAGGGagcggGCGCGCCGg -3' miRNA: 3'- -UGUCCUa---Ca---CGaCUUUCa----CGCGCGGC- -5' |
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| 29446 | 3' | -54.7 | NC_006151.1 | + | 100869 | 0.66 | 0.930819 |
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Target: 5'- gACuGGGccgccGUGaacCUGGAGGUGCuGCGCCGc -3' miRNA: 3'- -UGuCCUa----CAC---GACUUUCACG-CGCGGC- -5' |
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| 29446 | 3' | -54.7 | NC_006151.1 | + | 91755 | 0.66 | 0.930819 |
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Target: 5'- aGCGGGcucggGCgGgcGGUGUGCGCCa -3' miRNA: 3'- -UGUCCuaca-CGaCuuUCACGCGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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