Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29460 | 3' | -54.6 | NC_006151.1 | + | 35978 | 0.72 | 0.615833 |
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Target: 5'- -gCCGCcgCGGCCga-GGAAGAgGGCa -3' miRNA: 3'- aaGGUGuaGUCGGagaCCUUCUgCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 25359 | 0.73 | 0.59489 |
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Target: 5'- -aUCACAUCGGa---UGGGAGGCGGCg -3' miRNA: 3'- aaGGUGUAGUCggagACCUUCUGCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 98692 | 0.73 | 0.59489 |
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Target: 5'- -cCCGCGUCGuGCCcCcGGAgcAGACGGCg -3' miRNA: 3'- aaGGUGUAGU-CGGaGaCCU--UCUGCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 78747 | 0.73 | 0.563709 |
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Target: 5'- -cCCACGUCcGCCgUCUGcGccGAGGCGGCg -3' miRNA: 3'- aaGGUGUAGuCGG-AGAC-C--UUCUGCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 71618 | 0.74 | 0.492994 |
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Target: 5'- -aCCACcgCGGCCUUcucgccggGGggGACGGUc -3' miRNA: 3'- aaGGUGuaGUCGGAGa-------CCuuCUGCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 23501 | 0.77 | 0.382606 |
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Target: 5'- gUCCAgGUUGGCCUCcaccgacaGGGAGACGGUg -3' miRNA: 3'- aAGGUgUAGUCGGAGa-------CCUUCUGCCG- -5' |
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| 29460 | 3' | -54.6 | NC_006151.1 | + | 62157 | 1.1 | 0.002671 |
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Target: 5'- cUUCCACAUCAGCCUCUGGAAGACGGCg -3' miRNA: 3'- -AAGGUGUAGUCGGAGACCUUCUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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