Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29482 | 3' | -49.4 | NC_006151.1 | + | 47377 | 1.09 | 0.009483 |
Target: 5'- aAGUCAUAAAGUGGCACUCAGACAGCAc -3' miRNA: 3'- -UCAGUAUUUCACCGUGAGUCUGUCGU- -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 99243 | 0.69 | 0.970323 |
Target: 5'- aGGUCGUGGA--GGCGCUCGuGACcGCGg -3' miRNA: 3'- -UCAGUAUUUcaCCGUGAGU-CUGuCGU- -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 139503 | 0.68 | 0.980959 |
Target: 5'- cGGUCAUGGAGgacagcGGCAacagCAGcgGCAGCGa -3' miRNA: 3'- -UCAGUAUUUCa-----CCGUga--GUC--UGUCGU- -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 76067 | 0.67 | 0.989902 |
Target: 5'- gAG-CGUGAAG-GGCGC-CAG-CAGCGa -3' miRNA: 3'- -UCaGUAUUUCaCCGUGaGUCuGUCGU- -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 103814 | 0.67 | 0.990442 |
Target: 5'- uGUCGgcgGccGUGGCggcggccguggagauGCUCGGGCGGCu -3' miRNA: 3'- uCAGUa--UuuCACCG---------------UGAGUCUGUCGu -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 116105 | 0.66 | 0.994345 |
Target: 5'- cGUCGgcGA-UGGCGCggcgcuugUCGGGCAGCAc -3' miRNA: 3'- uCAGUauUUcACCGUG--------AGUCUGUCGU- -5' |
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29482 | 3' | -49.4 | NC_006151.1 | + | 84418 | 0.66 | 0.994345 |
Target: 5'- cGGUgAUAAGGcGGCGCgc-GGCGGCGc -3' miRNA: 3'- -UCAgUAUUUCaCCGUGaguCUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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