miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29482 3' -49.4 NC_006151.1 + 47377 1.09 0.009483
Target:  5'- aAGUCAUAAAGUGGCACUCAGACAGCAc -3'
miRNA:   3'- -UCAGUAUUUCACCGUGAGUCUGUCGU- -5'
29482 3' -49.4 NC_006151.1 + 76067 0.67 0.989902
Target:  5'- gAG-CGUGAAG-GGCGC-CAG-CAGCGa -3'
miRNA:   3'- -UCaGUAUUUCaCCGUGaGUCuGUCGU- -5'
29482 3' -49.4 NC_006151.1 + 84418 0.66 0.994345
Target:  5'- cGGUgAUAAGGcGGCGCgc-GGCGGCGc -3'
miRNA:   3'- -UCAgUAUUUCaCCGUGaguCUGUCGU- -5'
29482 3' -49.4 NC_006151.1 + 99243 0.69 0.970323
Target:  5'- aGGUCGUGGA--GGCGCUCGuGACcGCGg -3'
miRNA:   3'- -UCAGUAUUUcaCCGUGAGU-CUGuCGU- -5'
29482 3' -49.4 NC_006151.1 + 103814 0.67 0.990442
Target:  5'- uGUCGgcgGccGUGGCggcggccguggagauGCUCGGGCGGCu -3'
miRNA:   3'- uCAGUa--UuuCACCG---------------UGAGUCUGUCGu -5'
29482 3' -49.4 NC_006151.1 + 116105 0.66 0.994345
Target:  5'- cGUCGgcGA-UGGCGCggcgcuugUCGGGCAGCAc -3'
miRNA:   3'- uCAGUauUUcACCGUG--------AGUCUGUCGU- -5'
29482 3' -49.4 NC_006151.1 + 139503 0.68 0.980959
Target:  5'- cGGUCAUGGAGgacagcGGCAacagCAGcgGCAGCGa -3'
miRNA:   3'- -UCAGUAUUUCa-----CCGUga--GUC--UGUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.