Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29515 | 5' | -58.7 | NC_006151.1 | + | 68900 | 0.67 | 0.676731 |
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Target: 5'- -aGGacGGCCUCGCGGUcgcGCGGGCggaUCgCGa -3' miRNA: 3'- uaCC--CCGGAGUGCCA---CGUUCGa--AG-GC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 93744 | 0.66 | 0.746359 |
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Target: 5'- gAUGcGGGCCUCGuCGGgGCGcggGGCgUCCc -3' miRNA: 3'- -UAC-CCCGGAGU-GCCaCGU---UCGaAGGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 111856 | 0.68 | 0.625716 |
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Target: 5'- -gGGGGCgCUCGgGGUcGCAGGCcgUCUc -3' miRNA: 3'- uaCCCCG-GAGUgCCA-CGUUCGa-AGGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 112041 | 0.66 | 0.746359 |
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Target: 5'- cUGGGGCCg-GCGG-GCGGGCggCgGc -3' miRNA: 3'- uACCCCGGagUGCCaCGUUCGaaGgC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 121402 | 0.68 | 0.615495 |
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Target: 5'- uUGGGGCCU-GCGGcGCGGGCggggacgUCgCGg -3' miRNA: 3'- uACCCCGGAgUGCCaCGUUCGa------AG-GC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 131923 | 0.69 | 0.554685 |
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Target: 5'- --aGGGCCUcCGCGGUGCugccguAGCUggCCa -3' miRNA: 3'- uacCCCGGA-GUGCCACGu-----UCGAa-GGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 133615 | 0.66 | 0.765491 |
|
Target: 5'- -aGGGGCgUCugGGggucGC-GGCggCCGg -3' miRNA: 3'- uaCCCCGgAGugCCa---CGuUCGaaGGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 135943 | 0.66 | 0.726827 |
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Target: 5'- -cGGGGagccCCUCGCGGcGCGGGacgUCCu -3' miRNA: 3'- uaCCCC----GGAGUGCCaCGUUCga-AGGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 141273 | 0.69 | 0.584938 |
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Target: 5'- -cGGGGCCUCgGCGGcUGCGG---UCCGa -3' miRNA: 3'- uaCCCCGGAG-UGCC-ACGUUcgaAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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