Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29515 | 5' | -58.7 | NC_006151.1 | + | 10978 | 0.66 | 0.755979 |
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Target: 5'- -gGuGGGUgUCACGGUG-GAGCgggCCGg -3' miRNA: 3'- uaC-CCCGgAGUGCCACgUUCGaa-GGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 112041 | 0.66 | 0.746359 |
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Target: 5'- cUGGGGCCg-GCGG-GCGGGCggCgGc -3' miRNA: 3'- uACCCCGGagUGCCaCGUUCGaaGgC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 93744 | 0.66 | 0.746359 |
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Target: 5'- gAUGcGGGCCUCGuCGGgGCGcggGGCgUCCc -3' miRNA: 3'- -UAC-CCCGGAGU-GCCaCGU---UCGaAGGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 53977 | 0.66 | 0.736639 |
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Target: 5'- -cGGcGCCUcCGCGGgcgucgcgGCGAGCUcCCGg -3' miRNA: 3'- uaCCcCGGA-GUGCCa-------CGUUCGAaGGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 135943 | 0.66 | 0.726827 |
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Target: 5'- -cGGGGagccCCUCGCGGcGCGGGacgUCCu -3' miRNA: 3'- uaCCCC----GGAGUGCCaCGUUCga-AGGc -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 28261 | 0.67 | 0.716935 |
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Target: 5'- -cGGGGCCUCACacgugggccccgGGgacGCGGGC--CCGg -3' miRNA: 3'- uaCCCCGGAGUG------------CCa--CGUUCGaaGGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 21546 | 0.67 | 0.70697 |
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Target: 5'- -cGGGGCCggggcCugGGccucgGCGAGCccgCCGa -3' miRNA: 3'- uaCCCCGGa----GugCCa----CGUUCGaa-GGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 23971 | 0.67 | 0.686859 |
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Target: 5'- -aGGGGCCUCucuggccacgACGGUGCGgaccacgaggAGC--CCGa -3' miRNA: 3'- uaCCCCGGAG----------UGCCACGU----------UCGaaGGC- -5' |
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| 29515 | 5' | -58.7 | NC_006151.1 | + | 7290 | 1.06 | 0.002051 |
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Target: 5'- aAUGGGGCCUCACGGUGCAAGCUUCCGu -3' miRNA: 3'- -UACCCCGGAGUGCCACGUUCGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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