miRNA: 3'- -UGGGaUCUu---CCGCUCGG-------CGCUACGac -5'
miRNA: 3'- -UG-GGAUCUu--CCGc--UCGgCGCUACGAc -5'
miRNA: 3'- -UGGGauCUUC-CGCUCGGCGCua--CGac -5'
miRNA: 3'- -UGGGa-------UCUu---CCGCUCGGCGCua---CGac -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29522 | 5' | -57.9 | NC_006151.1 | + | 14931 | 0.66 | 0.798234 |
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Target: 5'- aGCCCgAGGccacGGCGGGCCcgccggcGCGGUGCc- -3' miRNA: 3'- -UGGGaUCUu---CCGCUCGG-------CGCUACGac -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 36681 | 0.66 | 0.799132 |
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Target: 5'- aGCgCCUGGAccuGGCcgcGGCgGCGGUGCUc -3' miRNA: 3'- -UG-GGAUCUu--CCGc--UCGgCGCUACGAc -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 26207 | 0.66 | 0.799132 |
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Target: 5'- gGCCC--GAGGcGCGGGCCGCGcgcgGCc- -3' miRNA: 3'- -UGGGauCUUC-CGCUCGGCGCua--CGac -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 117123 | 0.66 | 0.816768 |
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Target: 5'- gGCCCgccucacgGGGcggcGGCGGGCCGCGucgggGCg- -3' miRNA: 3'- -UGGGa-------UCUu---CCGCUCGGCGCua---CGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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