Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29613 | 5' | -51.9 | NC_006151.1 | + | 105350 | 0.68 | 0.945631 |
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Target: 5'- cGCGCUGCAGacggcgaccaUGgAGGUGCUG-GcGCUGc -3' miRNA: 3'- -CGCGACGUC----------AUaUCUAUGACgC-CGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 111277 | 0.7 | 0.879372 |
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Target: 5'- cGCGCggGCGGg--GGGUAC-GCGGcCUGg -3' miRNA: 3'- -CGCGa-CGUCauaUCUAUGaCGCC-GAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 120254 | 0.66 | 0.97724 |
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Target: 5'- cGCGCgugaGCuucg-AGGUGCUGCGGgaGc -3' miRNA: 3'- -CGCGa---CGucauaUCUAUGACGCCgaC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 121312 | 0.66 | 0.974634 |
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Target: 5'- gGCGCUGgAGcGUGGAg---GCGGCc- -3' miRNA: 3'- -CGCGACgUCaUAUCUaugaCGCCGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 125534 | 0.68 | 0.940901 |
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Target: 5'- cGCGgUGC-GUGgAGGUGCgcgGCGGCg- -3' miRNA: 3'- -CGCgACGuCAUaUCUAUGa--CGCCGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 127813 | 0.66 | 0.979643 |
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Target: 5'- gGCGCgagguccuUGCGGUucacguacGUGGcgGCgucGCGGCUGc -3' miRNA: 3'- -CGCG--------ACGUCA--------UAUCuaUGa--CGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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