Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29613 | 5' | -51.9 | NC_006151.1 | + | 73334 | 0.67 | 0.968784 |
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Target: 5'- gGCGCcGCGcGUGgcgaagAGGUuCUGCGGCa- -3' miRNA: 3'- -CGCGaCGU-CAUa-----UCUAuGACGCCGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 2589 | 0.67 | 0.965525 |
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Target: 5'- cGCGCUgGCGGU--AGGcGCgcgGCGGCa- -3' miRNA: 3'- -CGCGA-CGUCAuaUCUaUGa--CGCCGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 95918 | 0.67 | 0.965525 |
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Target: 5'- cGCGCgUGCAGUcgcccGAggGCgUGCGGUUGa -3' miRNA: 3'- -CGCG-ACGUCAuau--CUa-UG-ACGCCGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 103827 | 0.67 | 0.962033 |
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Target: 5'- gGCgGCgGCcGUGgAGAUGCUcggGCGGCUGc -3' miRNA: 3'- -CG-CGaCGuCAUaUCUAUGA---CGCCGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 49175 | 0.67 | 0.954328 |
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Target: 5'- cGCGCcGCG----GGGUGCUGCuGCUGa -3' miRNA: 3'- -CGCGaCGUcauaUCUAUGACGcCGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 61314 | 0.72 | 0.7653 |
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Target: 5'- cGCGCUGCAGguccagGGAgACgcccggccaGCGGCUGc -3' miRNA: 3'- -CGCGACGUCaua---UCUaUGa--------CGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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