Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29633 | 5' | -57.9 | NC_006151.1 | + | 72512 | 0.66 | 0.811487 |
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Target: 5'- aAGACacUGaCGCgGGCGGGUGC-CGUGu -3' miRNA: 3'- cUCUGc-AC-GUGgCCGCCCAUGaGCAU- -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 133796 | 0.66 | 0.802701 |
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Target: 5'- gGAGGCGUGCAgcauCCGGgugcacgucCGGGUGg-CGUAa -3' miRNA: 3'- -CUCUGCACGU----GGCC---------GCCCAUgaGCAU- -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 39466 | 0.66 | 0.802701 |
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Target: 5'- -cGcCGUGCGCUGGCccGGGUGCcgCGa- -3' miRNA: 3'- cuCuGCACGUGGCCG--CCCAUGa-GCau -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 97812 | 0.66 | 0.793762 |
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Target: 5'- cGGcCGUGCGCCGGuCGcGGUAgUUGg- -3' miRNA: 3'- cUCuGCACGUGGCC-GC-CCAUgAGCau -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 101227 | 0.66 | 0.793762 |
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Target: 5'- uGGACGggGC-CCGGCGGGgGCaUGUGc -3' miRNA: 3'- cUCUGCa-CGuGGCCGCCCaUGaGCAU- -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 54919 | 0.67 | 0.775461 |
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Target: 5'- --cGCGUGCA-CGGCGaGGUGCUggCGUGc -3' miRNA: 3'- cucUGCACGUgGCCGC-CCAUGA--GCAU- -5' |
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| 29633 | 5' | -57.9 | NC_006151.1 | + | 131859 | 0.72 | 0.487959 |
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Target: 5'- cGAGACGcGCGCCGGCGGcGUcgGgUCGc- -3' miRNA: 3'- -CUCUGCaCGUGGCCGCC-CA--UgAGCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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