Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29645 | 5' | -42.5 | NC_006151.1 | + | 88794 | 0.72 | 0.999396 |
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Target: 5'- cUCgacg--ACGACGGGcGCGAGCGCg -3' miRNA: 3'- uAGaauauaUGUUGUUCaCGCUUGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 90441 | 0.72 | 0.999396 |
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Target: 5'- cAUCagcgugAUGCAGCGGGUGgacuCGAGCGCg -3' miRNA: 3'- -UAGaaua--UAUGUUGUUCAC----GCUUGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 90682 | 0.72 | 0.999522 |
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Target: 5'- -gCUUGUcccccGCGGCGGGggcgGCGGACGCg -3' miRNA: 3'- uaGAAUAua---UGUUGUUCa---CGCUUGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 92552 | 0.69 | 0.999995 |
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Target: 5'- cUCg---GUGCAGCAGGUGgcCGAGuCGCu -3' miRNA: 3'- uAGaauaUAUGUUGUUCAC--GCUU-GCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 95616 | 0.7 | 0.99997 |
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Target: 5'- aGUCgggcccGUGCAGCGaggccaGGUGCGAgGCGCg -3' miRNA: 3'- -UAGaaua--UAUGUUGU------UCACGCU-UGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 115017 | 0.67 | 1 |
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Target: 5'- -gCUUGUGguagACgGugAGGUGCGGcagGCGCg -3' miRNA: 3'- uaGAAUAUa---UG-UugUUCACGCU---UGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 128222 | 0.66 | 1 |
|
Target: 5'- ------cGUGCGACGAGcucGUGGGCGCc -3' miRNA: 3'- uagaauaUAUGUUGUUCa--CGCUUGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 141407 | 0.72 | 0.999707 |
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Target: 5'- ----cGUGUccgGCGAUcGGUGCGGGCGCg -3' miRNA: 3'- uagaaUAUA---UGUUGuUCACGCUUGCG- -5' |
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| 29645 | 5' | -42.5 | NC_006151.1 | + | 141685 | 0.66 | 1 |
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Target: 5'- uGUCUgccucccacgGCGGCuGGcgGCGGACGCg -3' miRNA: 3'- -UAGAauaua-----UGUUGuUCa-CGCUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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