Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29649 | 3' | -55.8 | NC_006151.1 | + | 137411 | 0.66 | 0.888094 |
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Target: 5'- -cGGggGGCgcccUCGCcgcCGCCGCGGGGu -3' miRNA: 3'- gaCUaaCCG----AGCGaucGUGGUGCCCC- -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 53241 | 0.66 | 0.894826 |
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Target: 5'- gUGAgcaGGUucUCGCUGGCGCgCACGcGGc -3' miRNA: 3'- gACUaa-CCG--AGCGAUCGUG-GUGC-CCc -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 72812 | 0.66 | 0.894826 |
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Target: 5'- -cGAcgaGCUCGCcGGCGCCcACGGGc -3' miRNA: 3'- gaCUaacCGAGCGaUCGUGG-UGCCCc -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 92811 | 0.66 | 0.900687 |
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Target: 5'- gUGAcaaggucuUUGGCUCGCUgcugcGGCACgugaagaacgagaCGCGGGc -3' miRNA: 3'- gACU--------AACCGAGCGA-----UCGUG-------------GUGCCCc -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 122369 | 0.66 | 0.901326 |
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Target: 5'- gUGcacgGGCUCGaCgaggcGCGCCGCGGGc -3' miRNA: 3'- gACuaa-CCGAGC-Gau---CGUGGUGCCCc -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 128052 | 0.66 | 0.907592 |
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Target: 5'- -----cGGCggcaGCggcGGCugCGCGGGGg -3' miRNA: 3'- gacuaaCCGag--CGa--UCGugGUGCCCC- -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 116320 | 0.66 | 0.911837 |
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Target: 5'- aUGAcgccGcGCUCGCgcgggcccgcggcgUAGUACCGCGGGa -3' miRNA: 3'- gACUaa--C-CGAGCG--------------AUCGUGGUGCCCc -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 93438 | 0.66 | 0.913621 |
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Target: 5'- -aGGggGGCgagCGggGGUcgccGCCGCGGGGc -3' miRNA: 3'- gaCUaaCCGa--GCgaUCG----UGGUGCCCC- -5' |
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| 29649 | 3' | -55.8 | NC_006151.1 | + | 19270 | 0.66 | 0.913621 |
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Target: 5'- -----gGGCUCG-UGGCG-CGCGGGGc -3' miRNA: 3'- gacuaaCCGAGCgAUCGUgGUGCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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