Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29652 | 5' | -52.6 | NC_006152.1 | + | 3801 | 0.67 | 0.149203 |
Target: 5'- cGGcgaaCCCGggCACCACCGC-CACg -3' miRNA: 3'- aCCuuaaGGGUagGUGGUGGUGuGUG- -5' |
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29652 | 5' | -52.6 | NC_006152.1 | + | 1647 | 1.11 | 2.8e-05 |
Target: 5'- gUGGAAUUCCCAUCCACCACCACACACa -3' miRNA: 3'- -ACCUUAAGGGUAGGUGGUGGUGUGUG- -5' |
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29652 | 5' | -52.6 | NC_006152.1 | + | 814 | 0.71 | 0.06632 |
Target: 5'- gGGAAUauaCCCAgaaUCCACCACCuuAUugGCu -3' miRNA: 3'- aCCUUAa--GGGU---AGGUGGUGG--UGugUG- -5' |
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29652 | 5' | -52.6 | NC_006152.1 | + | 4018 | 0.67 | 0.134549 |
Target: 5'- uUGGAGguacacgUCCC-UCU-CCAuCCACACGCu -3' miRNA: 3'- -ACCUUa------AGGGuAGGuGGU-GGUGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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