Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2989 | 3' | -52.6 | NC_001493.1 | + | 59071 | 0.66 | 0.978719 |
Target: 5'- aUCACCGGUCGCGCgAaucuccacgaUGCAuuauuguuagcguucGAcCGAGu -3' miRNA: 3'- -AGUGGCUAGCGCGgU----------AUGU---------------CUaGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 46014 | 0.66 | 0.977768 |
Target: 5'- cUCGCCGAggagaggcugUCGCGC---ACAGAacuucgcgugcUCGAGg -3' miRNA: 3'- -AGUGGCU----------AGCGCGguaUGUCU-----------AGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 102481 | 0.66 | 0.977768 |
Target: 5'- uUCGCUGGUgGaaggugggGCCucgGCGGGUCGGGg -3' miRNA: 3'- -AGUGGCUAgCg-------CGGua-UGUCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 113322 | 0.66 | 0.977768 |
Target: 5'- aCACgggGAUCGUGCCGggucUGCGcGUCGAGu -3' miRNA: 3'- aGUGg--CUAGCGCGGU----AUGUcUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 106038 | 0.66 | 0.975255 |
Target: 5'- -aGCUGGUCGCGuUCGUGUGGGUCGGu -3' miRNA: 3'- agUGGCUAGCGC-GGUAUGUCUAGCUc -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 20993 | 0.66 | 0.975255 |
Target: 5'- gCGCCGcgcCGCGCCuccuCAGAuggguccugaUCGAGg -3' miRNA: 3'- aGUGGCua-GCGCGGuau-GUCU----------AGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 47431 | 0.66 | 0.97254 |
Target: 5'- uUCACCGAuacacUCGCGUCG-AUGGGggGAGg -3' miRNA: 3'- -AGUGGCU-----AGCGCGGUaUGUCUagCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 42771 | 0.66 | 0.969617 |
Target: 5'- -aACCGAUCGCcaCCAggaaGC-GAUCGGGg -3' miRNA: 3'- agUGGCUAGCGc-GGUa---UGuCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 82292 | 0.66 | 0.966478 |
Target: 5'- aCACCGGUCGCGagAUAaauCGGAUCu-- -3' miRNA: 3'- aGUGGCUAGCGCggUAU---GUCUAGcuc -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 132963 | 0.66 | 0.96516 |
Target: 5'- -gGCCGAUcaacucguccacgCGCGCCAgggUGCgggcacccccucugAGGUCGAGg -3' miRNA: 3'- agUGGCUA-------------GCGCGGU---AUG--------------UCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 17409 | 0.66 | 0.96516 |
Target: 5'- -gGCCGAUcaacucguccacgCGCGCCAgggUGCgggcacccccucugAGGUCGAGg -3' miRNA: 3'- agUGGCUA-------------GCGCGGU---AUG--------------UCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 71540 | 0.67 | 0.962064 |
Target: 5'- cCGCCguGAUCGCGaCCcggaugguaucagcGUAggcCAGAUCGAGg -3' miRNA: 3'- aGUGG--CUAGCGC-GG--------------UAU---GUCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 123656 | 0.67 | 0.959527 |
Target: 5'- aCAUCGAUgGaCGCCAUGguGAU-GAGu -3' miRNA: 3'- aGUGGCUAgC-GCGGUAUguCUAgCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 8102 | 0.67 | 0.959527 |
Target: 5'- aCAUCGAUgGaCGCCAUGguGAU-GAGu -3' miRNA: 3'- aGUGGCUAgC-GCGGUAUguCUAgCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 44569 | 0.67 | 0.959527 |
Target: 5'- cCGCCGcggacAUCGCGCgg-GCGGA-CGAGg -3' miRNA: 3'- aGUGGC-----UAGCGCGguaUGUCUaGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 56630 | 0.67 | 0.959527 |
Target: 5'- gCACCGAUC-CGCCcgagGCGGGcgCGAa -3' miRNA: 3'- aGUGGCUAGcGCGGua--UGUCUa-GCUc -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 56914 | 0.67 | 0.955705 |
Target: 5'- cCGCCGGUauaacaaaGCCAUccgccACGGGUCGGGg -3' miRNA: 3'- aGUGGCUAgcg-----CGGUA-----UGUCUAGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 43622 | 0.67 | 0.955705 |
Target: 5'- cUACgaGAUCG-GCCGcGCAGAUCGAc -3' miRNA: 3'- aGUGg-CUAGCgCGGUaUGUCUAGCUc -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 30926 | 0.67 | 0.955705 |
Target: 5'- aCGCCccUCGUGCCGUACAccgCGGGu -3' miRNA: 3'- aGUGGcuAGCGCGGUAUGUcuaGCUC- -5' |
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2989 | 3' | -52.6 | NC_001493.1 | + | 52686 | 0.67 | 0.951644 |
Target: 5'- aCGCgGGUUGaGCCAUGCGG-UCGAu -3' miRNA: 3'- aGUGgCUAGCgCGGUAUGUCuAGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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