Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30269 | 3' | -65.2 | NC_006296.2 | + | 5241 | 0.66 | 0.081133 |
Target: 5'- cACCCCCGCAccccaacacaGCGGaCCCUggUCACCCa -3' miRNA: 3'- cUGGGGGUGUcg--------CGUC-GGGG--GGUGGG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 4649 | 0.67 | 0.067892 |
Target: 5'- -cUCCCCACGGCuCcGCCCaCCGCCg -3' miRNA: 3'- cuGGGGGUGUCGcGuCGGGgGGUGGg -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 5193 | 0.68 | 0.062077 |
Target: 5'- aGGgCCCCACuccccCGCccCCCUCCGCCCg -3' miRNA: 3'- -CUgGGGGUGuc---GCGucGGGGGGUGGG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 4611 | 0.68 | 0.058471 |
Target: 5'- cGCaCCCCACcuGCcCuGCaCCCCCACCUg -3' miRNA: 3'- cUG-GGGGUGu-CGcGuCG-GGGGGUGGG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 5337 | 0.69 | 0.053439 |
Target: 5'- aGAUCUCCcCAGCcggGCAGCCCaaggCCACCa -3' miRNA: 3'- -CUGGGGGuGUCG---CGUCGGGg---GGUGGg -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 4945 | 0.74 | 0.019633 |
Target: 5'- aACCaCCCGCAGgGCccagggcccgGGCCCaCCACCCa -3' miRNA: 3'- cUGG-GGGUGUCgCG----------UCGGGgGGUGGG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 5108 | 0.76 | 0.012026 |
Target: 5'- aGAUCCCCaACAGCaGCA-CCCCCCACgCa -3' miRNA: 3'- -CUGGGGG-UGUCG-CGUcGGGGGGUGgG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 5009 | 0.79 | 0.006917 |
Target: 5'- cGCCCCacgCACAGCaGCAGagcaccuccagaCCCCCCACCCa -3' miRNA: 3'- cUGGGG---GUGUCG-CGUC------------GGGGGGUGGG- -5' |
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30269 | 3' | -65.2 | NC_006296.2 | + | 4545 | 1.09 | 1.5e-05 |
Target: 5'- aGACCCCCACAGCGCAGCCCCCCACCCc -3' miRNA: 3'- -CUGGGGGUGUCGCGUCGGGGGGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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