Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30316 | 5' | -57 | NC_006367.1 | + | 3945 | 0.67 | 0.085148 |
Target: 5'- aCGGGGUCAaGGAGccaGGACcaAGCGAa -3' miRNA: 3'- aGCCUCAGUgCCUC---CCUGcaUCGCUg -5' |
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30316 | 5' | -57 | NC_006367.1 | + | 4385 | 0.69 | 0.064977 |
Target: 5'- gUGGAGgcaUCACGGAGGGuACG-AGUcGCa -3' miRNA: 3'- aGCCUC---AGUGCCUCCC-UGCaUCGcUG- -5' |
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30316 | 5' | -57 | NC_006367.1 | + | 2668 | 0.69 | 0.062805 |
Target: 5'- cUGGuGGUCuuggcGCGGcugGGGGugGUGGUGGCa -3' miRNA: 3'- aGCC-UCAG-----UGCC---UCCCugCAUCGCUG- -5' |
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30316 | 5' | -57 | NC_006367.1 | + | 1461 | 1.1 | 1.6e-05 |
Target: 5'- gUCGGAGUCACGGAGGGACGUAGCGACg -3' miRNA: 3'- -AGCCUCAGUGCCUCCCUGCAUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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