Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30323 | 3' | -53.9 | NC_006383.2 | + | 4582 | 0.67 | 0.414109 |
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Target: 5'- uCCAG--GCGgggggcccCCCCCCGACcUGCCgGg -3' miRNA: 3'- -GGUUuuUGCa-------GGGGGGUUGuGCGGgC- -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 5376 | 0.68 | 0.364936 |
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Target: 5'- aCCGAGAGCcagccagagGUCCCCuCCAGuCcCGUCCa -3' miRNA: 3'- -GGUUUUUG---------CAGGGG-GGUU-GuGCGGGc -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 13834 | 0.68 | 0.355598 |
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Target: 5'- gUAGAGGaGUUCCCCCAucugguGCuuGCCCGa -3' miRNA: 3'- gGUUUUUgCAGGGGGGU------UGugCGGGC- -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 4996 | 0.7 | 0.271622 |
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Target: 5'- cCCAGGGAaggaGUCCCCCCccACACGgCg- -3' miRNA: 3'- -GGUUUUUg---CAGGGGGGu-UGUGCgGgc -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 5421 | 0.71 | 0.227376 |
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Target: 5'- cCCGAAGAggcccCGgcaaggCCCCCCAgcccgacgaccccuGCACGCaCCGg -3' miRNA: 3'- -GGUUUUU-----GCa-----GGGGGGU--------------UGUGCG-GGC- -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 4822 | 0.71 | 0.21036 |
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Target: 5'- cCCAagcAAGGCGgaCCCCCAACACgaaacuGCCCa -3' miRNA: 3'- -GGU---UUUUGCagGGGGGUUGUG------CGGGc -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 4546 | 0.74 | 0.138833 |
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Target: 5'- cCCGAugGGACGaggccggCCCCCCAACAaaCCCGg -3' miRNA: 3'- -GGUU--UUUGCa------GGGGGGUUGUgcGGGC- -5' |
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| 30323 | 3' | -53.9 | NC_006383.2 | + | 4680 | 1.12 | 0.000197 |
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Target: 5'- cCCAAAAACGUCCCCCCAACACGCCCGa -3' miRNA: 3'- -GGUUUUUGCAGGGGGGUUGUGCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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