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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30324 | 5' | -62.9 | NC_006383.2 | + | 4752 | 0.66 | 0.127662 |
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Target: 5'- aCCCCAaGGggGGA--CCCCCCaaaccaaagggaCCACc -3' miRNA: 3'- -GGGGUcCCuuCCUcaGGGGGG------------GGUGu -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 5332 | 0.66 | 0.124014 |
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Target: 5'- cCCCCGccaccGGGAcAGGGG-CUCCgCCaCCACAg -3' miRNA: 3'- -GGGGU-----CCCU-UCCUCaGGGG-GG-GGUGU- -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 9334 | 0.68 | 0.099307 |
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Target: 5'- aCCUCAGGGuacaagaccugauugAcugauaGGGAGUCCauggCCCCUACAg -3' miRNA: 3'- -GGGGUCCC---------------U------UCCUCAGGg---GGGGGUGU- -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 4808 | 0.72 | 0.046444 |
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Target: 5'- uCCCCGGuGGucccuuugguuuGGGGGGUCCCCCCUUGgGg -3' miRNA: 3'- -GGGGUC-CC------------UUCCUCAGGGGGGGGUgU- -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 4630 | 0.72 | 0.041011 |
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Target: 5'- gCCCGgcaggucGGGggGGGG-CCCCCCgCCugGa -3' miRNA: 3'- gGGGU-------CCCuuCCUCaGGGGGG-GGugU- -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 4466 | 0.75 | 0.025244 |
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Target: 5'- cCCCCA-GGAAGGGGccccacCCUCCCCCGCc -3' miRNA: 3'- -GGGGUcCCUUCCUCa-----GGGGGGGGUGu -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 4581 | 0.75 | 0.023027 |
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Target: 5'- gUCCAGGcGGGGGGcCCCCCCCCGa- -3' miRNA: 3'- gGGGUCCcUUCCUCaGGGGGGGGUgu -5' |
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| 30324 | 5' | -62.9 | NC_006383.2 | + | 4994 | 1.09 | 3.3e-05 |
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Target: 5'- gCCCCAGGGAAGGAGUCCCCCCCCACAc -3' miRNA: 3'- -GGGGUCCCUUCCUCAGGGGGGGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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