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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30327 | 5' | -54.7 | NC_006383.2 | + | 2648 | 0.7 | 0.192769 |
Target: 5'- uCGCAGaCUUGaGGACACUCcccACAGaCGCAu -3' miRNA: 3'- -GUGUC-GGAC-CCUGUGAGu--UGUC-GUGU- -5' |
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30327 | 5' | -54.7 | NC_006383.2 | + | 5451 | 0.68 | 0.272912 |
Target: 5'- cCGgGGCCUcuucgggGGGGCACUgGACGGgACu -3' miRNA: 3'- -GUgUCGGA-------CCCUGUGAgUUGUCgUGu -5' |
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30327 | 5' | -54.7 | NC_006383.2 | + | 9202 | 1.08 | 0.00018 |
Target: 5'- gCACAGCCUGGGACACUCAACAGCACAg -3' miRNA: 3'- -GUGUCGGACCCUGUGAGUUGUCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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