miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30407 5' -53.5 NC_006548.1 + 2248 0.97 0.006798
Target:  5'- cGCGCAAGGACUUCCugAGCACGCAGAa -3'
miRNA:   3'- -CGCGUUCCUGAAGGcaUCGUGCGUCU- -5'
30407 5' -53.5 NC_006548.1 + 9051 0.66 0.685592
Target:  5'- uGUGCAuccauugAGGGCUg-CGUggagcacGGUACGCAGAg -3'
miRNA:   3'- -CGCGU-------UCCUGAagGCA-------UCGUGCGUCU- -5'
30407 5' -53.5 NC_006548.1 + 19140 0.67 0.642241
Target:  5'- aGCGCuGGGAgaUCCG-AGCGggcuucUGCAGGa -3'
miRNA:   3'- -CGCGuUCCUgaAGGCaUCGU------GCGUCU- -5'
30407 5' -53.5 NC_006548.1 + 29026 0.67 0.630783
Target:  5'- aGCGCcuucGGugUcacCCGUGGCGaGCAGAc -3'
miRNA:   3'- -CGCGuu--CCugAa--GGCAUCGUgCGUCU- -5'
30407 5' -53.5 NC_006548.1 + 32581 0.67 0.653689
Target:  5'- uGCGCc-GGACUgggCUGaUGGCGUGCAGGa -3'
miRNA:   3'- -CGCGuuCCUGAa--GGC-AUCGUGCGUCU- -5'
30407 5' -53.5 NC_006548.1 + 33712 0.68 0.596459
Target:  5'- -aGCAcGGugUUCCGaggAGUACGCGa- -3'
miRNA:   3'- cgCGUuCCugAAGGCa--UCGUGCGUcu -5'
30407 5' -53.5 NC_006548.1 + 35003 0.69 0.496219
Target:  5'- -aGCAAuGGGCggCCGUAGCaaaGCGCAa- -3'
miRNA:   3'- cgCGUU-CCUGaaGGCAUCG---UGCGUcu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.