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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 30410 | 5' | -55.8 | NC_006548.1 | + | 3343 | 1.04 | 0.000854 |
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Target: 5'- gAUCGAGCUGGUGAUCACCUGCAGCAAc -3' miRNA: 3'- -UAGCUCGACCACUAGUGGACGUCGUU- -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 13515 | 0.73 | 0.168183 |
|
Target: 5'- gGUCGAGCUGGaacuguggcaguUGAUCACuCUGCuGCu- -3' miRNA: 3'- -UAGCUCGACC------------ACUAGUG-GACGuCGuu -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 25340 | 0.73 | 0.188036 |
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Target: 5'- uGUCGAGCUGGaaugGAUCgagaaggGCCUGCGcGCAc -3' miRNA: 3'- -UAGCUCGACCa---CUAG-------UGGACGU-CGUu -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 22020 | 0.73 | 0.183279 |
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Target: 5'- uAUCcAGCgcaucGGUGAUCACCgGCAGCGGg -3' miRNA: 3'- -UAGcUCGa----CCACUAGUGGaCGUCGUU- -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 21885 | 0.68 | 0.359925 |
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Target: 5'- gAUCGAGCaggaucagguUGGUGGUCugCUcgGUGGCAu -3' miRNA: 3'- -UAGCUCG----------ACCACUAGugGA--CGUCGUu -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 2152 | 0.66 | 0.478976 |
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Target: 5'- -aCGGGCuaccuUGGUGGacCGCUUGCAGCGu -3' miRNA: 3'- uaGCUCG-----ACCACUa-GUGGACGUCGUu -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 20726 | 0.66 | 0.48977 |
|
Target: 5'- cGUCGAGCUGGcguugGcgCuCCUGgAGCGc -3' miRNA: 3'- -UAGCUCGACCa----CuaGuGGACgUCGUu -5' |
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| 30410 | 5' | -55.8 | NC_006548.1 | + | 22744 | 0.66 | 0.500677 |
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Target: 5'- -aCGAGCUGcucGAUCGCCagcgugGCGGCGu -3' miRNA: 3'- uaGCUCGACca-CUAGUGGa-----CGUCGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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