Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30417 | 5' | -56.9 | NC_006548.1 | + | 5618 | 1.09 | 0.00034 |
Target: 5'- cCUGGCCUGCGCGUAGUAGGAGGCAACu -3' miRNA: 3'- -GACCGGACGCGCAUCAUCCUCCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 15201 | 0.72 | 0.194704 |
Target: 5'- -cGGCCUGCGCGUccgGGUugcuGGAcauacuguggauacGGCGGCc -3' miRNA: 3'- gaCCGGACGCGCA---UCAu---CCU--------------CCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 4806 | 0.7 | 0.278306 |
Target: 5'- -aGcGCCUGCGUG-AGUuGGAGGaCGGCu -3' miRNA: 3'- gaC-CGGACGCGCaUCAuCCUCC-GUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 15629 | 0.7 | 0.293105 |
Target: 5'- -cGGUCUGCGgGUAuuccgcGGAGGCAAUc -3' miRNA: 3'- gaCCGGACGCgCAUcau---CCUCCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 27873 | 0.69 | 0.341155 |
Target: 5'- cCUGGCgggugaggugCaGCGCaGUGGUGGccGAGGCGACg -3' miRNA: 3'- -GACCG----------GaCGCG-CAUCAUC--CUCCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 3034 | 0.68 | 0.39278 |
Target: 5'- -cGGCCgagcaguaccagGCGaa-GGUAGGAGGCAAg -3' miRNA: 3'- gaCCGGa-----------CGCgcaUCAUCCUCCGUUg -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 13739 | 0.67 | 0.423354 |
Target: 5'- -cGGCCUGCucgaugGCGUccagccGGUAGGAGaGCu-- -3' miRNA: 3'- gaCCGGACG------CGCA------UCAUCCUC-CGuug -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 25927 | 0.66 | 0.494881 |
Target: 5'- -cGGCCcGCGCGggccAGcAGGcGGUGACa -3' miRNA: 3'- gaCCGGaCGCGCa---UCaUCCuCCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 6648 | 0.66 | 0.505555 |
Target: 5'- -aGGCCaUGCaagaGCGUGGU-GGAGcCGACa -3' miRNA: 3'- gaCCGG-ACG----CGCAUCAuCCUCcGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 34963 | 0.69 | 0.309297 |
Target: 5'- gCUGGcCCUGCGCcUGGUcagccacgcacgugcGGAGGCGAg -3' miRNA: 3'- -GACC-GGACGCGcAUCAu--------------CCUCCGUUg -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 21293 | 0.69 | 0.332765 |
Target: 5'- -cGGCCUgGCGcCGgucgAGUAcGGcGGCAACg -3' miRNA: 3'- gaCCGGA-CGC-GCa---UCAU-CCuCCGUUG- -5' |
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30417 | 5' | -56.9 | NC_006548.1 | + | 36651 | 0.67 | 0.433197 |
Target: 5'- -aGGCCgGCGCGcugaucUGGUucuacgAGGAuugGGCAACa -3' miRNA: 3'- gaCCGGaCGCGC------AUCA------UCCU---CCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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