Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30419 | 5' | -53.1 | NC_006548.1 | + | 5595 | 0.66 | 0.687763 |
Target: 5'- -cGCAACUGGUUggcgAUGG-CGuCGAGCu -3' miRNA: 3'- caCGUUGACCAG----UGCCaGUuGCUCGu -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 6114 | 1.09 | 0.000929 |
Target: 5'- aGUGCAACUGGUCACGGUCAACGAGCAg -3' miRNA: 3'- -CACGUUGACCAGUGCCAGUUGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 13270 | 0.66 | 0.710469 |
Target: 5'- gGUGCcGCUcGGUagGCGGUCGauacAUGGGCGa -3' miRNA: 3'- -CACGuUGA-CCAg-UGCCAGU----UGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 17500 | 0.66 | 0.726166 |
Target: 5'- -gGCAACUGGacgacccaacugcgCACGcUCAuCGAGCAg -3' miRNA: 3'- caCGUUGACCa-------------GUGCcAGUuGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 18012 | 0.72 | 0.37741 |
Target: 5'- aUGCGGCUGGacgCuaugcacagcugcagGCGGUCAaggGCGAGCGa -3' miRNA: 3'- cACGUUGACCa--G---------------UGCCAGU---UGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 21444 | 0.73 | 0.329621 |
Target: 5'- aUGCAAgUGGUgGCGGcCGGCG-GCAg -3' miRNA: 3'- cACGUUgACCAgUGCCaGUUGCuCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 24314 | 0.68 | 0.607086 |
Target: 5'- -gGCcg--GGUCugGGcCGGCGAGCGc -3' miRNA: 3'- caCGuugaCCAGugCCaGUUGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 29347 | 0.67 | 0.630187 |
Target: 5'- aGUG-AGCUGGUCgGCGGcCAGcCGGGUAu -3' miRNA: 3'- -CACgUUGACCAG-UGCCaGUU-GCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 29892 | 0.71 | 0.41176 |
Target: 5'- cUGCAugACUGGUCcggcgcuguGCGGcacgCGACGGGCGg -3' miRNA: 3'- cACGU--UGACCAG---------UGCCa---GUUGCUCGU- -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 30290 | 0.66 | 0.737258 |
Target: 5'- -cGCGACcaGGUCugGGUUcgcggugaggaacugGACGAGUc -3' miRNA: 3'- caCGUUGa-CCAGugCCAG---------------UUGCUCGu -5' |
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30419 | 5' | -53.1 | NC_006548.1 | + | 34970 | 0.66 | 0.699151 |
Target: 5'- cUGCGcCUGGUCagccacgcacguGCGGa-GGCGAGCAa -3' miRNA: 3'- cACGUuGACCAG------------UGCCagUUGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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