Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30426 | 5' | -55.4 | NC_006548.1 | + | 14448 | 0.68 | 0.508258 |
Target: 5'- gCCggCGacgaccUGGaCgCACGCAGCGAGgCGCu -3' miRNA: 3'- -GGaaGCa-----ACC-GgGUGCGUCGUUC-GCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 7164 | 0.68 | 0.508258 |
Target: 5'- gCCUggggCGUgacgaGGCCCugGUgaucAGCAcGGCGUu -3' miRNA: 3'- -GGAa---GCAa----CCGGGugCG----UCGU-UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 2326 | 0.68 | 0.456092 |
Target: 5'- uCCUguUCGUcGG-CUGCGCGGCG-GCGCc -3' miRNA: 3'- -GGA--AGCAaCCgGGUGCGUCGUuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 28708 | 0.68 | 0.466301 |
Target: 5'- gUCUUCGUucuguuUGGCCaggCGCGCAGC-GGCu- -3' miRNA: 3'- -GGAAGCA------ACCGG---GUGCGUCGuUCGcg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36853 | 0.68 | 0.476627 |
Target: 5'- gCCUggCGcUGGUUCAuuCGCAGCGagucgaccGGCGCg -3' miRNA: 3'- -GGAa-GCaACCGGGU--GCGUCGU--------UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 13079 | 0.69 | 0.446004 |
Target: 5'- cCCUUCaGgacgcccUGGUCCAguacaaGCAGCAGGuCGCg -3' miRNA: 3'- -GGAAG-Ca------ACCGGGUg-----CGUCGUUC-GCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 7694 | 0.7 | 0.397522 |
Target: 5'- --cUCGgUGGUgUugGcCAGCAGGCGCc -3' miRNA: 3'- ggaAGCaACCGgGugC-GUCGUUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 20856 | 0.7 | 0.370106 |
Target: 5'- gCCUUC--UGGCgCG-GCAGCAGGCGg -3' miRNA: 3'- -GGAAGcaACCGgGUgCGUCGUUCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 20986 | 0.7 | 0.370106 |
Target: 5'- gCUUCGgcaGuGCUCACGCcauugcagcGCGAGCGCa -3' miRNA: 3'- gGAAGCaa-C-CGGGUGCGu--------CGUUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36461 | 0.71 | 0.34401 |
Target: 5'- cUCUUCGaaccgUGcagaCCCACGCGGCugagcAGCGCg -3' miRNA: 3'- -GGAAGCa----ACc---GGGUGCGUCGu----UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 25934 | 0.75 | 0.193343 |
Target: 5'- --gUCGUaacGGCCCGCGCgggccAGCAGGCGg -3' miRNA: 3'- ggaAGCAa--CCGGGUGCG-----UCGUUCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 28014 | 0.78 | 0.109259 |
Target: 5'- gCUUUGUguuggugcccagGGCCUugGCGGCAAGCGa -3' miRNA: 3'- gGAAGCAa-----------CCGGGugCGUCGUUCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 10188 | 1.14 | 0.000262 |
Target: 5'- gCCUUCGUUGGCCCACGCAGCAAGCGCg -3' miRNA: 3'- -GGAAGCAACCGGGUGCGUCGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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