Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30426 | 5' | -55.4 | NC_006548.1 | + | 25934 | 0.75 | 0.193343 |
Target: 5'- --gUCGUaacGGCCCGCGCgggccAGCAGGCGg -3' miRNA: 3'- ggaAGCAa--CCGGGUGCG-----UCGUUCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 25983 | 0.66 | 0.596257 |
Target: 5'- cCCUcagaUgGUUaaucGCCC-CGCAGCGGGgGCa -3' miRNA: 3'- -GGA----AgCAAc---CGGGuGCGUCGUUCgCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 28014 | 0.78 | 0.109259 |
Target: 5'- gCUUUGUguuggugcccagGGCCUugGCGGCAAGCGa -3' miRNA: 3'- gGAAGCAa-----------CCGGGugCGUCGUUCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 28708 | 0.68 | 0.466301 |
Target: 5'- gUCUUCGUucuguuUGGCCaggCGCGCAGC-GGCu- -3' miRNA: 3'- -GGAAGCA------ACCGG---GUGCGUCGuUCGcg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 28883 | 0.66 | 0.630018 |
Target: 5'- aCCUUCGggccaccGGCCaaaccgucgAgGCGGCcGGCGCc -3' miRNA: 3'- -GGAAGCaa-----CCGGg--------UgCGUCGuUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 32866 | 0.66 | 0.585054 |
Target: 5'- uUUUCGUgcugggGGCCuUGCGCuGCu-GCGCg -3' miRNA: 3'- gGAAGCAa-----CCGG-GUGCGuCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 33281 | 0.66 | 0.573892 |
Target: 5'- gCUUcCGUUGGCagaccuccggCCGCGaggAGCAugcGGCGCu -3' miRNA: 3'- gGAA-GCAACCG----------GGUGCg--UCGU---UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 34033 | 0.67 | 0.551727 |
Target: 5'- gCCUUCGacGGCCgaGCGCcacgguacAGCu-GCGCa -3' miRNA: 3'- -GGAAGCaaCCGGg-UGCG--------UCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 35939 | 0.67 | 0.540741 |
Target: 5'- aCCggcgaGUUGGCCgGCaggGUGGCGGcGCGCg -3' miRNA: 3'- -GGaag--CAACCGGgUG---CGUCGUU-CGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36461 | 0.71 | 0.34401 |
Target: 5'- cUCUUCGaaccgUGcagaCCCACGCGGCugagcAGCGCg -3' miRNA: 3'- -GGAAGCa----ACc---GGGUGCGUCGu----UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36590 | 0.67 | 0.551727 |
Target: 5'- gCCg-CGgccGGCaCCGCGguGCucGCGCg -3' miRNA: 3'- -GGaaGCaa-CCG-GGUGCguCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36853 | 0.68 | 0.476627 |
Target: 5'- gCCUggCGcUGGUUCAuuCGCAGCGagucgaccGGCGCg -3' miRNA: 3'- -GGAa-GCaACCGGGU--GCGUCGU--------UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 37477 | 0.66 | 0.618748 |
Target: 5'- ---cCGUU-GCCCAC-CAGCGAGCuagGCg -3' miRNA: 3'- ggaaGCAAcCGGGUGcGUCGUUCG---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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