Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30441 | 3' | -62.6 | NC_006548.1 | + | 14433 | 0.66 | 0.261383 |
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Target: 5'- cGCcGCUCCaGCUucuggccguagcgaUGCAcggcUGCaCGGCuGGGCa -3' miRNA: 3'- -CGuCGAGG-CGG--------------ACGU----ACG-GCCG-CCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 1195 | 0.66 | 0.263365 |
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Target: 5'- cCAGgagCCGgCUGCAaaGCCGGUacGGGCg -3' miRNA: 3'- cGUCga-GGCgGACGUa-CGGCCG--CCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 32485 | 0.66 | 0.268037 |
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Target: 5'- uGCAGCgCCGCgaacgGCAacggcggcgacggcUGCCGGCcaGGCu -3' miRNA: 3'- -CGUCGaGGCGga---CGU--------------ACGGCCGc-CCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 1243 | 0.66 | 0.270059 |
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Target: 5'- uCGGuCUuuGCUUGCGcGCgCGGUGcGGCa -3' miRNA: 3'- cGUC-GAggCGGACGUaCG-GCCGC-CCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 24029 | 0.66 | 0.276889 |
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Target: 5'- gGCAGUcgcgagCGCCUGCcagGCCGGgCGGuGUc -3' miRNA: 3'- -CGUCGag----GCGGACGua-CGGCC-GCC-CG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 27974 | 0.66 | 0.27689 |
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Target: 5'- aGCAGCUCaGCgaGUG-GCUGGaaaaGGGCc -3' miRNA: 3'- -CGUCGAGgCGgaCGUaCGGCCg---CCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 22768 | 0.66 | 0.27689 |
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Target: 5'- aGCGGCggagUCGCCuuUGCGgaucGCCgacgaGGCcGGGCg -3' miRNA: 3'- -CGUCGa---GGCGG--ACGUa---CGG-----CCG-CCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 27525 | 0.66 | 0.27689 |
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Target: 5'- gGCuGCUguUCGCCgGCAUcGCCGGUuacGGCc -3' miRNA: 3'- -CGuCGA--GGCGGaCGUA-CGGCCGc--CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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