Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30441 | 3' | -62.6 | NC_006548.1 | + | 23869 | 0.78 | 0.035036 |
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Target: 5'- cCGGgUCgCGCCaccgGCAUgGCCGGCGGGCu -3' miRNA: 3'- cGUCgAG-GCGGa---CGUA-CGGCCGCCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 16183 | 0.7 | 0.125484 |
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Target: 5'- -gAGCUgCGUCcGUGUGCCGGCGGa- -3' miRNA: 3'- cgUCGAgGCGGaCGUACGGCCGCCcg -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 16534 | 0.69 | 0.156063 |
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Target: 5'- aGguGCUCUGCC-GCA-GCCuGGUcgaucGGGCg -3' miRNA: 3'- -CguCGAGGCGGaCGUaCGG-CCG-----CCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 22684 | 0.69 | 0.160329 |
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Target: 5'- cGCAGUUCCGCUuccgGCAguUCGGCGaGCg -3' miRNA: 3'- -CGUCGAGGCGGa---CGUacGGCCGCcCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 1452 | 0.69 | 0.173764 |
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Target: 5'- aGCAGUUCCug--GCcgG-CGGCGGGCa -3' miRNA: 3'- -CGUCGAGGcggaCGuaCgGCCGCCCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 21824 | 0.68 | 0.188193 |
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Target: 5'- cGguGCguugucgauaUCCGCCUGCGgcaCCGGCGcguccaGGCc -3' miRNA: 3'- -CguCG----------AGGCGGACGUac-GGCCGC------CCG- -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 29614 | 0.68 | 0.19683 |
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Target: 5'- -gAGUUCCGCCUGCGguggaaagcgcuccUGC-GGCGGa- -3' miRNA: 3'- cgUCGAGGCGGACGU--------------ACGgCCGCCcg -5' |
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| 30441 | 3' | -62.6 | NC_006548.1 | + | 24029 | 0.66 | 0.276889 |
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Target: 5'- gGCAGUcgcgagCGCCUGCcagGCCGGgCGGuGUc -3' miRNA: 3'- -CGUCGag----GCGGACGua-CGGCC-GCC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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