Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30462 | 3' | -52 | NC_006548.1 | + | 17175 | 0.71 | 0.485669 |
Target: 5'- aAUCUGCGAgUCGuucaaccUCGgccCGGCCCGUu -3' miRNA: 3'- -UAGACGUUaAGC-------AGCauuGCCGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 2067 | 0.71 | 0.486747 |
Target: 5'- cGUCgGCGAUgUUGUCGUcGAC-GCCCGCg -3' miRNA: 3'- -UAGaCGUUA-AGCAGCA-UUGcCGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 28022 | 0.7 | 0.576093 |
Target: 5'- uUCUGCcg--CGguUCGgcGCGGCCCuGCa -3' miRNA: 3'- uAGACGuuaaGC--AGCauUGCCGGG-CG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 2884 | 0.68 | 0.657018 |
Target: 5'- cGUCgaagGCGAUgUUGUCGgccAUGGCCCGa -3' miRNA: 3'- -UAGa---CGUUA-AGCAGCau-UGCCGGGCg -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 27004 | 0.68 | 0.657018 |
Target: 5'- cGUCgGCGAgucCGcCGaucagcaGACGGCCCGCa -3' miRNA: 3'- -UAGaCGUUaa-GCaGCa------UUGCCGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 25946 | 1.11 | 0.000919 |
Target: 5'- gAUCUGCAAUUCGUCGUAACGGCCCGCg -3' miRNA: 3'- -UAGACGUUAAGCAGCAUUGCCGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 28156 | 0.69 | 0.622258 |
Target: 5'- uUCUGCAGgcgGUUGaguuCGGCCUGCa -3' miRNA: 3'- uAGACGUUaagCAGCauu-GCCGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 21836 | 0.67 | 0.736631 |
Target: 5'- -gUUGUAGUUCGUCGguGCGuugucgauauccGCCUGCg -3' miRNA: 3'- uaGACGUUAAGCAGCauUGC------------CGGGCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 21444 | 0.67 | 0.736631 |
Target: 5'- --aUGCAa---GUgGUGGCGGCCgGCg -3' miRNA: 3'- uagACGUuaagCAgCAUUGCCGGgCG- -5' |
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30462 | 3' | -52 | NC_006548.1 | + | 36809 | 0.66 | 0.790263 |
Target: 5'- cUCUGCGAgcgUCGcCGagcugguGCGGCUCaGCa -3' miRNA: 3'- uAGACGUUa--AGCaGCau-----UGCCGGG-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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