Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30470 | 3' | -56.4 | NC_006548.1 | + | 19132 | 0.66 | 0.505889 |
Target: 5'- --gGCAGCGCAGCgcugggagauCCGAgCGgGCUUc -3' miRNA: 3'- guaCGUCGCGUCG----------GGUUgGUgCGAAc -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 28767 | 0.66 | 0.505889 |
Target: 5'- --cGCAaCGCAGCCCGGCCcuUGCc-- -3' miRNA: 3'- guaCGUcGCGUCGGGUUGGu-GCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 16981 | 0.66 | 0.505889 |
Target: 5'- gCAUGCAGgGCcGCCagUAGCUugGCcUUGu -3' miRNA: 3'- -GUACGUCgCGuCGG--GUUGGugCG-AAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 22065 | 0.66 | 0.495128 |
Target: 5'- --cGguGCGaCGGCCCAG-CACGCc-- -3' miRNA: 3'- guaCguCGC-GUCGGGUUgGUGCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 1066 | 0.66 | 0.494058 |
Target: 5'- cCAUGUcgacgacccagccGGCGCAGCUCAGCauCGCUg- -3' miRNA: 3'- -GUACG-------------UCGCGUCGGGUUGguGCGAac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 1261 | 0.66 | 0.473927 |
Target: 5'- --cGCGGUGCGGCa-GGCUugGCUUu -3' miRNA: 3'- guaCGUCGCGUCGggUUGGugCGAAc -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 23758 | 0.66 | 0.473927 |
Target: 5'- uUcgGCGGCGCcGCCCA--CGUGCUUGa -3' miRNA: 3'- -GuaCGUCGCGuCGGGUugGUGCGAAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 13078 | 0.66 | 0.473927 |
Target: 5'- --aGCAGCGCAGCgUcACCcucgGCGCg-- -3' miRNA: 3'- guaCGUCGCGUCGgGuUGG----UGCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 1921 | 0.67 | 0.463498 |
Target: 5'- gGUGCccgauccccuGGUGCAGCC--GCgCGCGCUUGc -3' miRNA: 3'- gUACG----------UCGCGUCGGguUG-GUGCGAAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 21935 | 0.67 | 0.461426 |
Target: 5'- --cGCGGCGUAGCUauuggggcugaaGACCA-GCUUGg -3' miRNA: 3'- guaCGUCGCGUCGGg-----------UUGGUgCGAAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 29577 | 0.67 | 0.443009 |
Target: 5'- gGUGaCAGCaGCAGCUCccgucACCAUGCUg- -3' miRNA: 3'- gUAC-GUCG-CGUCGGGu----UGGUGCGAac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 29825 | 0.67 | 0.443009 |
Target: 5'- --gGCGGcCGCAaCCCAGCCGgGCaUGa -3' miRNA: 3'- guaCGUC-GCGUcGGGUUGGUgCGaAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 7204 | 0.67 | 0.432957 |
Target: 5'- gAUGcCAGCGacaGGCCCAgcucuGCCGCgGCUg- -3' miRNA: 3'- gUAC-GUCGCg--UCGGGU-----UGGUG-CGAac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 36688 | 0.67 | 0.432957 |
Target: 5'- uCAUGCGGCGCuGGCaCCGuauCgACGCa-- -3' miRNA: 3'- -GUACGUCGCG-UCG-GGUu--GgUGCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 2731 | 0.68 | 0.403622 |
Target: 5'- --gGCGGCGCAGggCAAgCACGCUg- -3' miRNA: 3'- guaCGUCGCGUCggGUUgGUGCGAac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 27861 | 0.68 | 0.394126 |
Target: 5'- gGUGCAGCGCAGUgguggCCGagGCgACGCc-- -3' miRNA: 3'- gUACGUCGCGUCG-----GGU--UGgUGCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 36371 | 0.68 | 0.384776 |
Target: 5'- -cUGCAgaugaacuacGCGCuGCUCAGCCGCGUggGa -3' miRNA: 3'- guACGU----------CGCGuCGGGUUGGUGCGaaC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 20118 | 0.68 | 0.376488 |
Target: 5'- gCAUGCcgucGCGCaccuccagacccuuGGCCCAGCCAUaggccggcgcauugaGCUUGg -3' miRNA: 3'- -GUACGu---CGCG--------------UCGGGUUGGUG---------------CGAAC- -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 16576 | 0.68 | 0.375575 |
Target: 5'- -cUGguGaCGCGGuCCCAGCCcACGCg-- -3' miRNA: 3'- guACguC-GCGUC-GGGUUGG-UGCGaac -5' |
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30470 | 3' | -56.4 | NC_006548.1 | + | 32464 | 0.68 | 0.375575 |
Target: 5'- --gGCGGCGaCGGCugCCGGCCAgGCUg- -3' miRNA: 3'- guaCGUCGC-GUCG--GGUUGGUgCGAac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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