Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 30472 | 3' | -51 | NC_006548.1 | + | 4805 | 0.71 | 0.531294 |
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Target: 5'- gCGCcuGCgUGAguugGAgGACGGCUUCACc -3' miRNA: 3'- gGCGu-CG-ACUaa--CUgUUGCCGAAGUG- -5' |
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| 30472 | 3' | -51 | NC_006548.1 | + | 12960 | 0.72 | 0.476114 |
|
Target: 5'- gCGCAGCcGuggUGACcACGGCUcCGCa -3' miRNA: 3'- gGCGUCGaCua-ACUGuUGCCGAaGUG- -5' |
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| 30472 | 3' | -51 | NC_006548.1 | + | 17496 | 0.73 | 0.423932 |
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Target: 5'- gCCGCuGCUGGgu--CGACGGCUgaUCGCc -3' miRNA: 3'- -GGCGuCGACUaacuGUUGCCGA--AGUG- -5' |
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| 30472 | 3' | -51 | NC_006548.1 | + | 37756 | 0.74 | 0.394313 |
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Target: 5'- gCGCAGCUGAUcUGuCGuCGGCcUCACc -3' miRNA: 3'- gGCGUCGACUA-ACuGUuGCCGaAGUG- -5' |
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| 30472 | 3' | -51 | NC_006548.1 | + | 5594 | 0.75 | 0.356966 |
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Target: 5'- gCGCAaCUGGUUGGCGAUGGCgUCGa -3' miRNA: 3'- gGCGUcGACUAACUGUUGCCGaAGUg -5' |
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| 30472 | 3' | -51 | NC_006548.1 | + | 30941 | 1.12 | 0.00099 |
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Target: 5'- cCCGCAGCUGAUUGACAACGGCUUCACu -3' miRNA: 3'- -GGCGUCGACUAACUGUUGCCGAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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