Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30491 | 5' | -59.2 | NC_006548.1 | + | 21014 | 0.66 | 0.391871 |
Target: 5'- cGCCGUCGCCGgauuucucggaaaagUCCaccugUUUgGGCAGUUc -3' miRNA: 3'- aCGGCGGCGGU---------------AGG-----AAAgCCGUCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 8197 | 0.66 | 0.388217 |
Target: 5'- aGCgG-CGCUAUCCagUUcCGGCAGCUc -3' miRNA: 3'- aCGgCgGCGGUAGGa-AA-GCCGUCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 2439 | 0.66 | 0.385491 |
Target: 5'- aGCCGgcucggauggaaaaCCGCCggCCUggCGGguGCg- -3' miRNA: 3'- aCGGC--------------GGCGGuaGGAaaGCCguCGac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 24990 | 0.66 | 0.37918 |
Target: 5'- cGCCGCCgacGCCAaCCUa--GGUGGCUu -3' miRNA: 3'- aCGGCGG---CGGUaGGAaagCCGUCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 20337 | 0.66 | 0.37918 |
Target: 5'- gGgCGCCGUCAUCC--UCGGCgaAGUUc -3' miRNA: 3'- aCgGCGGCGGUAGGaaAGCCG--UCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 3459 | 0.66 | 0.370285 |
Target: 5'- cGCCGUgGCCuucaccagGUUCUUUCGaGCguccaGGCUGg -3' miRNA: 3'- aCGGCGgCGG--------UAGGAAAGC-CG-----UCGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 21461 | 0.66 | 0.370285 |
Target: 5'- cGCC-CCgGCCGUUCUgcCGGUAGcCUGa -3' miRNA: 3'- aCGGcGG-CGGUAGGAaaGCCGUC-GAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 36272 | 0.67 | 0.344466 |
Target: 5'- gUGCgGUCGggcgggGUCCagUUCGGCGGCUGg -3' miRNA: 3'- -ACGgCGGCgg----UAGGa-AAGCCGUCGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 4653 | 0.67 | 0.33946 |
Target: 5'- uUGCUGCCGUgGaaaagcaccuggaguUCCgcUUCGGCcgGGCUGg -3' miRNA: 3'- -ACGGCGGCGgU---------------AGGa-AAGCCG--UCGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 37408 | 0.67 | 0.336152 |
Target: 5'- gGCCugGCCGCUAg----UCGGCAGCg- -3' miRNA: 3'- aCGG--CGGCGGUaggaaAGCCGUCGac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 29681 | 0.67 | 0.319967 |
Target: 5'- nGCgGCUGCCGguag--UGGCGGCUGg -3' miRNA: 3'- aCGgCGGCGGUaggaaaGCCGUCGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 16714 | 0.67 | 0.304373 |
Target: 5'- cGCagGCCGCCGUCCUggucauagCGGaAGCg- -3' miRNA: 3'- aCGg-CGGCGGUAGGAaa------GCCgUCGac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 29744 | 0.68 | 0.282088 |
Target: 5'- aGCCaGCCGCCA-CUac-CGGCAGCc- -3' miRNA: 3'- aCGG-CGGCGGUaGGaaaGCCGUCGac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 33704 | 0.68 | 0.282088 |
Target: 5'- cGCUGUCGCUGUCCUgcCGGCAa--- -3' miRNA: 3'- aCGGCGGCGGUAGGAaaGCCGUcgac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 21158 | 0.68 | 0.267962 |
Target: 5'- cGCCGcCCGCCGuaucgugaUCCa--UGGCAGCUc -3' miRNA: 3'- aCGGC-GGCGGU--------AGGaaaGCCGUCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 21385 | 0.68 | 0.261116 |
Target: 5'- cGCCgGCCGCCAcCaCUUgcaucaUCGGCuucGCUGa -3' miRNA: 3'- aCGG-CGGCGGUaG-GAA------AGCCGu--CGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 22614 | 0.68 | 0.261116 |
Target: 5'- gGCCGUCGaUCAggcucUCCUccgcCGGCGGCUGu -3' miRNA: 3'- aCGGCGGC-GGU-----AGGAaa--GCCGUCGAC- -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 27291 | 0.69 | 0.247852 |
Target: 5'- gGCUGCCGCC------UCGGCAGCg- -3' miRNA: 3'- aCGGCGGCGGuaggaaAGCCGUCGac -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 25291 | 0.69 | 0.223 |
Target: 5'- uUGCCGCCcaCCAUUCgcuggUCGcGCAGCUc -3' miRNA: 3'- -ACGGCGGc-GGUAGGaa---AGC-CGUCGAc -5' |
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30491 | 5' | -59.2 | NC_006548.1 | + | 35875 | 0.71 | 0.184617 |
Target: 5'- aUGCCGgCGCgAUCCgcgcggcCGGCGGCg- -3' miRNA: 3'- -ACGGCgGCGgUAGGaaa----GCCGUCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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