Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30491 | 5' | -59.2 | NC_006548.1 | + | 19396 | 0.71 | 0.179636 |
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Target: 5'- aGCuCGCUGCCGUCCagcUGGUAGCg- -3' miRNA: 3'- aCG-GCGGCGGUAGGaaaGCCGUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 18396 | 0.71 | 0.174774 |
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Target: 5'- cGCCGCUGCCAcCCgaacCGGCuGCg- -3' miRNA: 3'- aCGGCGGCGGUaGGaaa-GCCGuCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 32424 | 0.73 | 0.125012 |
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Target: 5'- cGCCGuuGCCGUUCg--CGGC-GCUGc -3' miRNA: 3'- aCGGCggCGGUAGGaaaGCCGuCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 18521 | 0.74 | 0.108431 |
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Target: 5'- cGUgGCCGCCggGUCCaugUCGGCAcGCUGu -3' miRNA: 3'- aCGgCGGCGG--UAGGaa-AGCCGU-CGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 29650 | 0.75 | 0.08866 |
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Target: 5'- gUGCCGCUGCCAgcggugCCggugCGGCAGgUGc -3' miRNA: 3'- -ACGGCGGCGGUa-----GGaaa-GCCGUCgAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 552 | 1.07 | 0.000285 |
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Target: 5'- cUGCCGCCGCCAUCCUUUCGGCAGCUGu -3' miRNA: 3'- -ACGGCGGCGGUAGGAAAGCCGUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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