Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30491 | 5' | -59.2 | NC_006548.1 | + | 29744 | 0.68 | 0.282088 |
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Target: 5'- aGCCaGCCGCCA-CUac-CGGCAGCc- -3' miRNA: 3'- aCGG-CGGCGGUaGGaaaGCCGUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 32424 | 0.73 | 0.125012 |
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Target: 5'- cGCCGuuGCCGUUCg--CGGC-GCUGc -3' miRNA: 3'- aCGGCggCGGUAGGaaaGCCGuCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 33704 | 0.68 | 0.282088 |
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Target: 5'- cGCUGUCGCUGUCCUgcCGGCAa--- -3' miRNA: 3'- aCGGCGGCGGUAGGAaaGCCGUcgac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 35875 | 0.71 | 0.184617 |
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Target: 5'- aUGCCGgCGCgAUCCgcgcggcCGGCGGCg- -3' miRNA: 3'- -ACGGCgGCGgUAGGaaa----GCCGUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 36272 | 0.67 | 0.344466 |
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Target: 5'- gUGCgGUCGggcgggGUCCagUUCGGCGGCUGg -3' miRNA: 3'- -ACGgCGGCgg----UAGGa-AAGCCGUCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 37408 | 0.67 | 0.336152 |
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Target: 5'- gGCCugGCCGCUAg----UCGGCAGCg- -3' miRNA: 3'- aCGG--CGGCGGUaggaaAGCCGUCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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